ArticleJournal of neurochemistry2010
Role of astrocytic leptin receptor subtypes on leptin permeation across hCMEC/D3 human brain endothelial cells.
Article in Journal of neurochemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 28 citations in OpenAlex.
- Discovery of a Novel Dual Targeting Peptide for Human Glioma: FromAdvanced pharmaceutical bulletin · 2024Article
- Recent Advances in the Knowledge of the Mechanisms of Leptin Physiology and Actions in Neurological and Metabolic Pathologies.International journal of molecular sciences · 2023Review
- Amino Acid Nanofibers Improve Glycemia and Confer Cognitive Therapeutic Efficacy to Bound Insulin.Pharmaceutics · 2021Article
- Article
- Deletion of astrocytic BMAL1 results in metabolic imbalance and shorter lifespan in mice.Glia · 2020Article
- Fertility in classical galactosaemia, a study of N-glycan, hormonal and inflammatory gene interactions.Orphanet journal of rare diseases · 2018Article
- β1-integrin-matrix interactions modulate cerebral microvessel endothelial cell tight junction expression and permeability.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2018Article
- Limitations of the hCMEC/D3 cell line as a model for Aβ clearance by the human blood-brain barrier.Journal of neuroscience research · 2017Article
- Direct modulation of GFAP-expressing glia in the arcuate nucleus bi-directionally regulates feeding.eLife · 2016Article
- Differential effects of leptin and adiponectin in endothelial angiogenesis.Journal of diabetes research · 2015Review
- Role of non-neuronal cells in body weight and appetite control.Frontiers in endocrinology · 2015Review
- Saturable leptin transport across the BBB persists in EAE mice.Journal of molecular neuroscience : MN · 2013Article
- Upregulation of astrocytic leptin receptor in mice with experimental autoimmune encephalomyelitis.Journal of molecular neuroscience : MN · 2013Article
- Multicellular self-assembled spheroidal model of the blood brain barrier.Scientific reports · 2013Article
- The effects of a high-energy diet on hippocampal-dependent discrimination performance and blood-brain barrier integrity differ for diet-induced obese and diet-resistant rats.Physiology & behavior · 2012Article
- Leptin action on nonneuronal cells in the CNS: potential clinical applications.Annals of the New York Academy of Sciences · 2012Review
- C-reactive protein increases BBB permeability: implications for obesity and neuroinflammation.Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology · 2012Article
- Interendothelial claudin-5 expression depends on cerebral endothelial cell-matrix adhesion by β(1)-integrins.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2011Article
- Effects of cell-type specific leptin receptor mutation on leptin transport across the BBB.Peptides · 2011Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 4 countries.
Funding
Abstract
Astrocytic leptin receptors (ObR) can be up-regulated in conditions such as adult-onset obesity. To determine whether the levels and subtypes of astrocytic ObR modulate leptin transport, we co-cultured hCMEC/D3 human brain endothelial cells and C6 astrocytoma cells in the Transwell system, and tested leptin permeation from apical to basolateral chambers. In comparison with hCMEC alone, co-culture of C6 cells reduced the permeability of paracellular markers and leptin. Unexpectedly, ObRb over-expression in C6 cells increased leptin permeation whereas ObRa over-expression showed no effect when compared with the control group of pcDNA-transfected C6 cells. By contrast, the paracellular permeability to the sodium fluorescein control was unchanged by over-expression of ObR subtypes. Leptin remained intact after crossing the monolayer as shown by HPLC and acid precipitation, and this was not affected by C6 cell co-culture or the over-expression of different ObR subtypes. Thus, increased expression of ObRb (and to a lesser extent ObRe) in C6 cells specifically increased the permeation of leptin across the hCMEC monolayer. Consistent with the evidence that the most apparent regulatory changes of ObR during obesity and inflammation occur in astrocytes, the results indicate that astrocytes actively regulate leptin transport across the blood-brain barrier, a mechanism independent of reduction of paracellular permeability.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.