ArticlePloS one2010
NtGNL1 plays an essential role in pollen tube tip growth and orientation likely via regulation of post-Golgi trafficking.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Visualizing Pollen and Pollen Tube Development Using Fluorescence Microscopy.Methods in molecular biology (Clifton, N.J.) · 2026Article
- NtLLG4-mediated unconventional polar exocytosis of NtPPME1 coordinates cell wall rigidity and membrane dynamics to control pollen tube integrity.Science advances · 2025Article
- Global identification of key genes for pollen germination in rice through high-throughput screening and gene editing.Journal of integrative plant biology · 2025Article
- Long-Distance Movement ofPlants (Basel, Switzerland) · 2023Article
- Dissection and ultramicroscopic observation of an apical pollen tube of Pyrus.Plant reproduction · 2022Article
- The legacy of kinesins in the pollen tube 30 years later.Cytoskeleton (Hoboken, N.J.) · 2022Review
- Importance of organellar proteins, protein translocation and vesicle transport routes for pollen development and function.Plant reproduction · 2016Review
- NtGNL1a ARF-GEF acts in endocytosis in tobacco cells.BMC plant biology · 2015Article
- Organization and regulation of the actin cytoskeleton in the pollen tube.Frontiers in plant science · 2014Review
- Antisense oligodeoxynucleotide inhibition as an alternative and convenient method for gene function analysis in pollen tubes.PloS one · 2013Article
- Endoplasmic reticulum- and Golgi-localized phospholipase A2 plays critical roles in Arabidopsis pollen development and germination.The Plant cell · 2011Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTobacco GNOM LIKE 1 (NtGNL1), a new member of the Big/GBF family, is characterized by a sec 7 domain. Thus, we proposed that NtGNL1 may function in regulating pollen tube growth for vesicle trafficking. METHODOLOGY/PRINCIPAL
findingsTo test this hypothesis, we used an RNAi technique to down-regulate NtGNL1 expression and found that pollen tube growth and orientation were clearly inhibited. Cytological observations revealed that both timing and behavior of endocytosis was disrupted, and endosome trafficking to prevacuolar compartments (PVC) or multivesicular bodies (MVB) was altered in pollen tube tips. Moreover, NtGNL1 seemed to partially overlap with Golgi bodies, but clearly colocalized with putative late endosome compartments. We also observed that in such pollen tubes, the Golgi apparatus disassembled and fused with the endoplasmic reticulum, indicating abnormal post-Golgi trafficking. During this process, actin organization was also remodeled. CONCLUSIONS/SIGNIFICANCE: Thus, we revealed that NtGNL1 is essential for pollen tube growth and orientation and it likely functions via stabilizing the structure of the Golgi apparatus and ensuring post-Golgi trafficking.
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