ArticlePloS one2010
Protein crosslinking by transglutaminase controls cuticle morphogenesis in Drosophila.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 46 citations in OpenAlex.
- Drosophila Transglutaminase preserves the integrity of muscle attachments with and without mechanical strain.Journal of cell science · 2026Article
- Single-cell profiling of the developing embryonic heart in Drosophila.Development (Cambridge, England) · 2023Article
- Systemic coagulopathy promotes host lethality in a new Drosophila tumor model.Current biology : CB · 2023Article
- Physical and Chemical Barriers in the Larval Midgut Confer Developmental Resistance to Virus Infection inViruses · 2021Article
- RNAi-Mediated Knockdown of Imaginal Disc Growth Factors (IDGFs) Genes Causes Developmental Malformation and Mortality in Melon Fly,Frontiers in genetics · 2021Article
- Imaginal Disc Growth Factor 6 (Idgf6) Is Involved in Larval and Adult Wing Development inFrontiers in genetics · 2020Article
- The putative C-type lectin Schlaff ensures epidermal barrier compactness in Drosophila.Scientific reports · 2019Article
- Article
- Transglutaminase-catalyzed incorporation of polyamines masks the DNA-binding region of the transcription factor Relish.The Journal of biological chemistry · 2017Article
- Arthropod Innate Immune Systems and Vector-Borne Diseases.Biochemistry · 2017Review
- Properties of the cuticular proteins of Anopheles gambiae as revealed by serial extraction of adults.PloS one · 2017Article
- RNA Interference Directed against the Transglutaminase Gene Triggers Dysbiosis of Gut Microbiota in Drosophila.The Journal of biological chemistry · 2016Article
- Chitinases and Imaginal disc growth factors organize the extracellular matrix formation at barrier tissues in insects.Scientific reports · 2016Article
- Crosslinking of a Peritrophic Matrix Protein Protects Gut Epithelia from Bacterial Exotoxins.PLoS pathogens · 2015Article
- Inhibition of transglutaminase exacerbates polyglutamine-induced neurotoxicity by increasing the aggregation of mutant ataxin-3 in an SCA3 Drosophila model.Neurotoxicity research · 2015Article
- Dihydroisoxazole inhibitors of Anopheles gambiae seminal transglutaminase AgTG3.Malaria journal · 2014Article
- Integrated proteomic and transcriptomic analysis of the Aedes aegypti eggshell.BMC developmental biology · 2014Article
- AP-1/Fos-TGase2 axis mediates wounding-induced Plasmodium falciparum killing in Anopheles gambiae.The Journal of biological chemistry · 2013Article
- Characterization of Anopheles gambiae transglutaminase 3 (AgTG3) and its native substrate Plugin.The Journal of biological chemistry · 2013Article
- Serine proteolytic pathway activation reveals an expanded ensemble of wound response genes in Drosophila.PloS one · 2013Article
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transglutaminase (TG) plays important and diverse roles in mammals, such as blood coagulation and formation of the skin barrier, by catalyzing protein crosslinking. In invertebrates, TG is known to be involved in immobilization of invading pathogens at sites of injury. Here we demonstrate that Drosophila TG is an important enzyme for cuticle morphogenesis. Although TG activity was undetectable before the second instar larval stage, it dramatically increased in the third instar larval stage. RNA interference (RNAi) of the TG gene caused a pupal semi-lethal phenotype and abnormal morphology. Furthermore, TG-RNAi flies showed a significantly shorter life span than their counterparts, and approximately 90% of flies died within 30 days after eclosion. Stage-specific TG-RNAi before the third instar larval stage resulted in cuticle abnormality, but the TG-RNAi after the late pupal stage did not, indicating that TG plays a key role at or before the early pupal stage. Immediately following eclosion, acid-extractable protein from wild-type wings was nearly all converted to non-extractable protein due to wing maturation, whereas several proteins remained acid-extractable in the mature wings of TG-RNAi flies. We identified four proteins--two cuticular chitin-binding proteins, larval serum protein 2, and a putative C-type lectin-as TG substrates. RNAi of their corresponding genes caused a lethal phenotype or cuticle abnormality. Our results indicate that TG-dependent protein crosslinking in Drosophila plays a key role in cuticle morphogenesis and sclerotization.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.