ArticleBioconjugate chemistry2009
STED nanoscopy in living cells using Fluorogen Activating Proteins.
Article in Bioconjugate chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed.
- Azetidinyl Malachite Green: a superior fluorogen-activating protein probe for live-cell and dynamic SIM imaging.Chemical science · 2025Article
- Experimental Approaches to Visualize Effector Protein Translocation During Host-Pathogen Interactions.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- The potential of fluorogenicity for single molecule FRET and DyeCycling.QRB discovery · 2024Review
- Transient Fluorescence Labeling: Low Affinity-High Benefits.International journal of molecular sciences · 2021Review
- Chemogenetic Tags with Probe Exchange for Live-Cell Fluorescence Microscopy.ACS chemical biology · 2021Article
- Genetically Encoded Activators of Small Molecules for Imaging and Drug Delivery.Angewandte Chemie (International ed. in English) · 2020Review
- Next-Generation Fluorogen-Based Reporters and Biosensors for Advanced Bioimaging.International journal of molecular sciences · 2019Review
- Single-molecule labeling for studying trafficking of renal transporters.American journal of physiology. Renal physiology · 2018Review
- The application of 'kisser' probes for resolving the distribution and microenvironment of membrane proteins in situ.Journal of neurogenetics · 2018Article
- Fluorogen-activating proteins: beyond classical fluorescent proteins.Acta pharmaceutica Sinica. B · 2018Review
- Fluorogenic Labeling Strategies for Biological Imaging.International journal of molecular sciences · 2017Review
- Photostable and photoswitching fluorescent dyes for super-resolution imaging.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2017Review
- Super-resolution Imaging of Live Bacteria Cells Using a Genetically Directed, Highly Photostable Fluoromodule.Journal of the American Chemical Society · 2016Article
- Rapid, specific, no-wash, far-red fluorogen activation in subcellular compartments by targeted fluorogen activating proteins.ACS chemical biology · 2015Article
- Advances in chemical labeling of proteins in living cells.Cell and tissue research · 2015Review
- Translation. An RNA biosensor for imaging the first round of translation from single cells to living animals.Science (New York, N.Y.) · 2015Article
- A bifunctional converter: fluorescein quenching scFv/fluorogen activating protein for photostability and improved signal to noise in fluorescence experiments.Bioconjugate chemistry · 2014Article
- Fluorogen-activating scFv biosensors target surface markers on live cells via streptavidin or single-chain avidin.Molecular biotechnology · 2014Article
- Localization microscopy using noncovalent fluorogen activation by genetically encoded fluorogen-activating proteins.Chemphyschem : a European journal of chemical physics and physical chemistry · 2014Article
- Malachite green mediates homodimerization of antibody VL domains to form a fluorescent ternary complex with singular symmetric interfaces.Journal of molecular biology · 2013Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
We demonstrate the effectiveness of a genetically encoded Malachite Green (MG) binding fluorogen activating protein (FAP) for live cell stimulated emission depletion nanoscopy (STED). Both extracellular and intracellular FAPs were tested in living cells using fluorogens with either membrane expressed FAP or as an intracellular FAP-actin fusion. Structures with FWHM of 110-122nm were observed. Depletion data however suggests a resolution of 70nm with the given instrument.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.