ArticlePloS one2010
eXtraembryonic ENdoderm (XEN) stem cells produce factors that activate heart formation.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 35 citations in OpenAlex.
- Tissue-intrinsic beta-catenin signals antagonize Nodal-driven anterior visceral endoderm differentiation.Nature communications · 2024Article
- The Trophoblast Compartment Helps Maintain Embryonic Pluripotency and Delays Differentiation towards Cardiomyocytes.International journal of molecular sciences · 2023Article
- Tbx5 overexpression in embryoid bodies increases TAK1 expression but does not enhance the differentiation of sinoatrial node cardiomyocytes.Biology open · 2023Article
- MLL3/MLL4 methyltransferase activities control early embryonic development and embryonic stem cell differentiation in a lineage-selective manner.Nature genetics · 2023Article
- Dissecting the Complexity of Early Heart Progenitor Cells.Journal of cardiovascular development and disease · 2021Review
- Control of cardiomyocyte differentiation timing by intercellular signaling pathways.Seminars in cell & developmental biology · 2021Review
- TAK1/Map3k7 enhances differentiation of cardiogenic endoderm from mouse embryonic stem cells.Journal of molecular and cellular cardiology · 2019Article
- Characterization of porcine extraembryonic endoderm cells.Cell proliferation · 2019Article
- In vitro generation of mouse polarized embryo-like structures from embryonic and trophoblast stem cells.Nature protocols · 2018Article
- Capturing and Interconverting Embryonic Cell Fates in a Dish.Current topics in developmental biology · 2018Review
- Overexpression of Map3k7 activates sinoatrial node-like differentiation in mouse ES-derived cardiomyocytes.PloS one · 2017Article
- Efficient derivation of extraembryonic endoderm stem cell lines from mouse postimplantation embryos.Scientific reports · 2016Article
- Gata6 potently initiates reprograming of pluripotent and differentiated cells to extraembryonic endoderm stem cells.Genes & development · 2015Article
- CstF-64 is necessary for endoderm differentiation resulting in cardiomyocyte defects.Stem cell research · 2014Article
- A close look at the mammalian blastocyst: epiblast and primitive endoderm formation.Cellular and molecular life sciences : CMLS · 2014Review
- Hhex and Cer1 mediate the Sox17 pathway for cardiac mesoderm formation in embryonic stem cells.Stem cells (Dayton, Ohio) · 2014Article
- Derivation of extraembryonic endoderm stem (XEN) cells from mouse embryos and embryonic stem cells.Nature protocols · 2013Article
- Early cardiac development: a view from stem cells to embryos.Cardiovascular research · 2012Review
- Conversion from mouse embryonic to extra-embryonic endoderm stem cells reveals distinct differentiation capacities of pluripotent stem cell states.Development (Cambridge, England) · 2012Article
- Induction of cardiomyogenesis in human embryonic stem cells by human embryonic stem cell-derived definitive endoderm.Stem cells and development · 2012Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundInitial specification of cardiomyocytes in the mouse results from interactions between the extraembryonic anterior visceral endoderm (AVE) and the nascent mesoderm. However the mechanism by which AVE activates cardiogenesis is not well understood, and the identity of specific cardiogenic factors in the endoderm remains elusive. Most mammalian studies of the cardiogenic potential of the endoderm have relied on the use of cell lines that are similar to the heart-inducing AVE. These include the embryonal-carcinoma-derived cell lines, END2 and PYS2. The recent development of protocols to isolate eXtraembryonic ENdoderm (XEN) stem cells, representing the extraembryonic endoderm lineage, from blastocyst stage mouse embryos offers new tools for the genetic dissection of cardiogenesis. METHODOLOGY/PRINCIPAL
findingsHere, we demonstrate that XEN cell-conditioned media (CM) enhances cardiogenesis during Embryoid Body (EB) differentiation of mouse embryonic stem (ES) cells in a manner comparable to PYS2-CM and END2-CM. Addition of CM from each of these three cell lines enhanced the percentage of EBs that formed beating areas, but ultimately, only XEN-CM and PYS2-CM increased the total number of cardiomyocytes that formed. Furthermore, our observations revealed that both contact-independent and contact-dependent factors are required to mediate the full cardiogenic potential of the endoderm. Finally, we used gene array comparison to identify factors in these cell lines that could mediate their cardiogenic potential. CONCLUSIONS/SIGNIFICANCE: These studies represent the first step in the use of XEN cells as a molecular genetic tool to study cardiomyocyte differentiation. Not only are XEN cells functionally similar to the heart-inducing AVE, but also can be used for the genetic dissection of the cardiogenic potential of AVE, since they can be isolated from both wild type and mutant blastocysts. These studies further demonstrate the importance of both contact-dependent and contact-independent factors in cardiogenesis and identify potential heart-inducing proteins in the endoderm.
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