ArticlePLoS genetics2010
Evidence for a Xer/dif system for chromosome resolution in archaea.
Article in PLoS genetics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 51 citations in OpenAlex.
- Ecological and evolutionary implications of a mobile genetic element-rich haloarchaeon with unique osmotic resilience.Microbiology spectrum · 2026Article
- Chromosomal domain formation by archaeal SMC, a roadblock protein, and DNA structure.Nature communications · 2025Article
- Article
- Recruitment of Mobile Genetic Elements for Diverse Cellular Functions in Prokaryotes.Frontiers in molecular biosciences · 2022Review
- Archaeal tyrosine recombinases.FEMS microbiology reviews · 2021Review
- Sequence analysis of tyrosine recombinases allows annotation of mobile genetic elements in prokaryotic genomes.Molecular systems biology · 2021Article
- Review
- Pervasive Suicidal Integrases in Deep-Sea Archaea.Molecular biology and evolution · 2020Article
- Characterization of the Chromosome Dimer Resolution Site in Caulobacter crescentus.Journal of bacteriology · 2019Article
- A novel family of tyrosine integrases encoded by the temperate pleolipovirus SNJ2.Nucleic acids research · 2018Article
- Flipping chromosomes in deep-sea archaea.PLoS genetics · 2017Article
- Xer Site Specific Recombination: Double and Single Recombinase Systems.Frontiers in microbiology · 2017Review
- Structural snapshots of Xer recombination reveal activation by synaptic complex remodeling and DNA bending.eLife · 2016Article
- Site-Specific Recombination by SSV2 Integrase: Substrate Requirement and Domain Functions.Journal of virology · 2015Article
- Comparative genomics reveals conserved positioning of essential genomic clusters in highly rearranged Thermococcales chromosomes.Biochimie · 2015Article
- Extracellular Vesicles of the Hyperthermophilic Archaeon "Thermococcus onnurineus" NA1T.Applied and environmental microbiology · 2015Article
- Genome Sequence of a Hyperthermophilic Archaeon, Thermococcus nautili 30-1, That Produces Viral Vesicles.Genome announcements · 2014Article
- Archaeal chromosome biology.Journal of molecular microbiology and biotechnology · 2014Review
- The Xer/dif site-specific recombination system of Campylobacter jejuni.Molecular genetics and genomics : MGG · 2013Article
- The cell cycle of archaea.Nature reviews. Microbiology · 2013Review
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Homologous recombination events between circular chromosomes, occurring during or after replication, can generate dimers that need to be converted to monomers prior to their segregation at cell division. In Escherichia coli, chromosome dimers are converted to monomers by two paralogous site-specific tyrosine recombinases of the Xer family (XerC/D). The Xer recombinases act at a specific dif site located in the replication termination region, assisted by the cell division protein FtsK. This chromosome resolution system has been predicted in most Bacteria and further characterized for some species. Archaea have circular chromosomes and an active homologous recombination system and should therefore resolve chromosome dimers. Most archaea harbour a single homologue of bacterial XerC/D proteins (XerA), but not of FtsK. Therefore, the role of XerA in chromosome resolution was unclear. Here, we have identified dif-like sites in archaeal genomes by using a combination of modeling and comparative genomics approaches. These sites are systematically located in replication termination regions. We validated our in silico prediction by showing that the XerA protein of Pyrococcus abyssi specifically recombines plasmids containing the predicted dif site in vitro. In contrast to the bacterial system, XerA can recombine dif sites in the absence of protein partners. Whereas Archaea and Bacteria use a completely different set of proteins for chromosome replication, our data strongly suggest that XerA is most likely used for chromosome resolution in Archaea.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.