ArticleNature communications2010
Imaging the nanoscale organization of peptidoglycan in living Lactococcus lactis cells.
Article in Nature communications, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 139 citations in OpenAlex.
- Review
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- Surveying membrane landscapes: a new look at the bacterial cell surface.Nature reviews. Microbiology · 2023Review
- PBP2b Mutations Improve the Growth of Phage-ResistantApplied and environmental microbiology · 2023Article
- Nanoscale clustering of mycobacterial ligands and DC-SIGN host receptors are key determinants for pathogen recognition.Science advances · 2023Article
- Class A Penicillin-Binding Protein C Is Responsible for Stress Response by Regulation of Peptidoglycan Assembly in Clavibacter michiganensis.Microbiology spectrum · 2022Article
- Study of tribological properties of human buccal epithelium cell membranes using probe microscopy.Scientific reports · 2022Article
- Opposing Effects of PhoPQ and PmrAB on the Properties ofBiochemistry · 2021Article
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- Structural Visualization of Septum Formation in Staphylococcus warneri Using Atomic Force Microscopy.Journal of bacteriology · 2020Article
- Cell wall homeostasis in lactic acid bacteria: threats and defences.FEMS microbiology reviews · 2020Review
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- A biphasic growth model for cell pole elongation in mycobacteria.Nature communications · 2020Article
- Microbial adhesion and ultrastructure from the single-molecule to the single-cell levels by Atomic Force Microscopy.Cell surface (Amsterdam, Netherlands) · 2019Review
- In vitro single-cell dissection revealing the interior structure of cable bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Atomic Force Microscopy of Side Wall and Septa Peptidoglycan FromFrontiers in microbiology · 2018Article
- Beyond the bulk: disclosing the life of single microbial cells.FEMS microbiology reviews · 2017Review
- Article
- Imaging modes of atomic force microscopy for application in molecular and cell biology.Nature nanotechnology · 2017Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The spatial organization of peptidoglycan, the major constituent of bacterial cell-walls, is an important, yet still unsolved issue in microbiology. In this paper, we show that the combined use of atomic force microscopy and cell wall mutants is a powerful platform for probing the nanoscale architecture of cell wall peptidoglycan in living Gram-positive bacteria. Using topographic imaging, we found that Lactococcus lactis wild-type cells display a smooth, featureless surface morphology, whereas mutant strains lacking cell wall exopolysaccharides feature 25-nm-wide periodic bands running parallel to the short axis of the cell. In addition, we used single-molecule recognition imaging to show that parallel bands are made of peptidoglycan. Our data, obtained for the first time on living ovococci, argue for an architectural feature of the cell wall in the plane perpendicular to the long axis of the cell. The non-invasive live cell experiments presented here open new avenues for understanding the architecture and assembly of peptidoglycan in Gram-positive bacteria.
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Registered trials
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