ArticleProceedings of the National Academy of Sciences of the United States of America2010
Hypoxia abrogates antichlamydial properties of IFN-γ in human fallopian tube cells in vitro and ex vivo.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 65 citations in OpenAlex.
- The Role of the Vaginal Microbiome in Gynecological Diseases: Mechanistic Insights and Emerging Interventions.Biology · 2026Review
- Chlamydia trachomatis disrupts host metabolism in primary cervical epithelial cells.World journal of microbiology & biotechnology · 2025Article
- Insights into Chlamydia Development and Host Cells Response.Microorganisms · 2024Review
- Contributions of diverse models of the female reproductive tract to the study of Chlamydia trachomatis-host interactions.Current opinion in microbiology · 2024Review
- Intracellular lifestyle of Chlamydia trachomatis and host-pathogen interactions.Nature reviews. Microbiology · 2023Review
- Sorangicin A Is Active againstAntibiotics (Basel, Switzerland) · 2023Article
- A Member of an Ancient Family of Bacterial Amino Acids Transporters Contributes to Chlamydia Nutritional Virulence and Immune Evasion.Infection and immunity · 2023Article
- Association ofFrontiers in cellular and infection microbiology · 2023Article
- Mechanisms controlling bacterial infection in myeloid cells under hypoxic conditions.Cellular and molecular life sciences : CMLS · 2021Review
- Comparison of In Vitro Chlamydia muridarum Infection Under Aerobic and Anaerobic Conditions.Current microbiology · 2020Article
- Infection-driven activation of transglutaminase 2 boosts glucose uptake and hexosamine biosynthesis in epithelial cells.The EMBO journal · 2020Article
- Chronic Inflammatory Diseases at Secondary Sites Ensuing Urogenital or PulmonaryMicroorganisms · 2020Review
- The Interaction Between Microorganisms, Metabolites, and Immune System in the Female Genital Tract Microenvironment.Frontiers in cellular and infection microbiology · 2020Review
- Murine Endometrial Organoids to ModelFrontiers in cellular and infection microbiology · 2020Article
- Elaborations on Corallopyronin A as a Novel Treatment Strategy Against Genital Chlamydial Infections.Frontiers in microbiology · 2019Article
- The role of tryptophan metabolism in postpartum depression.Metabolic brain disease · 2018Review
- Review
- Negative Impact of Hypoxia on Tryptophan 2,3-Dioxygenase Function.Mediators of inflammation · 2016Article
- Catalytic activity of human indoleamine 2,3-dioxygenase (hIDO1) at low oxygen.Archives of biochemistry and biophysics · 2015Article
- Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
IFN-γ has an important role in the adaptive immune response against intracellular pathogens. In urogenital tract (UGT) infections with the obligate intracellular pathogen Chlamydia trachomatis, IFN-γ-mediated control of chlamydial growth implies the JAK-STAT signaling cascades and subsequent induction of the indoleamine 2,3-dioxygenase (IDO). As oxygen concentrations in the UGT are low under physiological conditions (O(2) < 5%) and further decrease during an inflammatory process, we wondered whether antibacterial properties of IFN-γ are maintained under hypoxic conditions. Using primary cells that were isolated from human fallopian tubes and an ex vivo human fallopian tube model (HFTM), we found that even high IFN-γ concentrations (200 units/mL) were not sufficient to limit growth of C. trachomatis under hypoxia. Reduced antibacterial activity of IFN-γ under hypoxia was restricted to the urogenital serovars D and L(2), but was not observed with the ocular serovar A. Impaired effectiveness of IFN-γ on chlamydial growth under hypoxia was accompanied by reduced phosphorylation of Stat-1 on Tyr701 and diminished IDO activity. This study shows that IFN-γ effector functions on intracellular C. trachomatis depend on the environmental oxygen supply, which could explain inadequate bacterial clearance and subsequent chronic infections eventually occurring in the UGT of women.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.