ArticleProceedings of the National Academy of Sciences of the United States of America2010
Generation of induced pluripotent stem cells using site-specific integration with phage integrase.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 48 citations in OpenAlex.
- Deciphering reprogramming efficiency in human induced pluripotent stem cells: insights from the generation of 150 cell lines.Frontiers in immunology · 2025Article
- Recent advances in CRISPR-Cas9-based genome insertion technologies.Molecular therapy. Nucleic acids · 2024Review
- Application of Human Stem Cells to Model Genetic Sensorineural Hearing Loss and Meniere Disease.Cells · 2023Review
- The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System.Journal of visualized experiments : JoVE · 2017Article
- Choices for Induction of Pluripotency: Recent Developments in Human Induced Pluripotent Stem Cell Reprogramming Strategies.Stem cell reviews and reports · 2016Review
- The Use of Induced Pluripotent Stem Cells for the Study and Treatment of Liver Diseases.Current protocols in toxicology · 2016Review
- Simple derivation of transgene-free iPS cells by a dual recombinase approach.Molecular biotechnology · 2014Article
- New applications for phage integrases.Journal of molecular biology · 2014Review
- Improved site-specific recombinase-based method to produce selectable marker- and vector-backbone-free transgenic cells.Scientific reports · 2014Article
- Article
- Expanding the scope of site-specific recombinases for genetic and metabolic engineering.Biotechnology and bioengineering · 2014Review
- Recombinase-mediated reprogramming and dystrophin gene addition in mdx mouse induced pluripotent stem cells.PloS one · 2014Article
- Zinc finger nuclease-expressing baculoviral vectors mediate targeted genome integration of reprogramming factor genes to facilitate the generation of human induced pluripotent stem cells.Stem cells translational medicine · 2013Article
- Targeting human embryonic stem cells with quantum dot-conjugated phages.Scientific reports · 2013Article
- Blood cell-derived induced pluripotent stem cells free of reprogramming factors generated by Sendai viral vectors.Stem cells translational medicine · 2013Article
- Generation and genetic engineering of human induced pluripotent stem cells using designed zinc finger nucleases.Stem cells and development · 2013Article
- A site-specific recombinase-based method to produce antibiotic selectable marker free transgenic cattle.PloS one · 2013Article
- Efficient generation of rat induced pluripotent stem cells using a non-viral inducible vector.PloS one · 2013Article
- Streptomyces temperate bacteriophage integration systems for stable genetic engineering of actinomycetes (and other organisms).Journal of industrial microbiology & biotechnology · 2012Review
- Investigating cellular identity and manipulating cell fate using induced pluripotent stem cells.Stem cell research & therapy · 2012Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To date, a large number of reports have described reprogramming many somatic cell types into induced pluripotent stem (iPS) cells, using different numbers of transcription factors and devising alternate methods of introducing the transcription factor genes or proteins into the somatic cells. Here, we describe a method using bacteriophage ΦC31 integrase to reprogram mouse embryonic fibroblasts and human amniotic fluid cells into iPS cells. These iPS cells showed morphology, surface antigens, gene expression, and epigenetic states similar to ES cells and formed teratomas with three germ layers in nonobese diabetic/severely compromised immunodeficient mice. Importantly, these iPS cells have only a single integration site in each cell line. The locations of integration favor the intergenic regions, and their distances from the adjacent genes extended from several hundred to >1 million bp. The effect of the insertion on the expression of these genes can be studied by RT-PCR. No insertion into microRNA gene loci was detected. Hence, it is possible to select cells in which adjacent gene functions are not affected, or the inserts can be removed if necessary. We conclude that phage integrase-mediated site-specific recombination can produce iPS cells that have undisturbed endogenous gene function and could be safe for future human therapeutic application.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.