ArticleProceedings of the National Academy of Sciences of the United States of America2010
Domain structure of the DEMETER 5-methylcytosine DNA glycosylase.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 78 citations in OpenAlex.
- DMN-seq enriches DNA hypomethylated regions for biomarker discovery using 5-methylcytosine glycosylase.Genome biology · 2026Article
- Identification of DNA Methyltransferase/Demethylase Genes and 5-Azacytidine's Impact on β-Elemene and Methylation inBiology · 2025Article
- Control of DNA demethylation by superoxide anion in plant stem cells.Nature chemical biology · 2025Article
- INCREASED DNA METHYLATION 3 forms a potential chromatin remodelling complex with HAIRPLUS to regulate DNA methylation and trichome development in tomato.The Plant journal : for cell and molecular biology · 2025Article
- Genome-Wide Analysis of DNA Demethylases in Land Plants and Their Expression Pattern in Rice.Plants (Basel, Switzerland) · 2024Article
- The pivotal ripening gene SlDML2 participates in regulating disease resistance in tomato.Plant biotechnology journal · 2023Article
- Base Excision DNA Repair in Plants:International journal of molecular sciences · 2023Review
- Molecular basis of the plant ROS1-mediated active DNA demethylation.Nature plants · 2023Article
- Insights into the molecular mechanisms of CRISPR/Cas9-mediated gene targeting at multiple loci in Arabidopsis.Plant physiology · 2022Article
- Geminiviruses employ host DNA glycosylases to subvert DNA methylation-mediated defense.Nature communications · 2022Article
- Recent Advances on DNA Base Flipping: A General Mechanism for Writing, Reading, and Erasing DNA Modifications.Advances in experimental medicine and biology · 2022Article
- Identification and Characterization of DNA Demethylase Genes and Their Association With Thermal Stress in Wheat (Frontiers in genetics · 2022Article
- Targeting Induced Local Lesions in the WheatFrontiers in nutrition · 2022Article
- Review
- Application of 5-Methylcytosine DNA Glycosylase to the Quantitative Analysis of DNA Methylation.International journal of molecular sciences · 2021Article
- The Arabidopsis active demethylase ROS1eLife · 2021Article
- Article
- Active DNA Demethylation in Plants.International journal of molecular sciences · 2019Review
- The catalytic core of DEMETER guides active DNA demethylation inProceedings of the National Academy of Sciences of the United States of America · 2019Article
- CRISPR/Cas9-mediated gene targeting in Arabidopsis using sequential transformation.Nature communications · 2018Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
DNA glycosylases initiate the base excision repair (BER) pathway by excising damaged, mismatched, or otherwise modified bases. Animals and plants independently evolved active BER-dependent DNA demethylation mechanisms important for epigenetic reprogramming. One such DNA demethylation mechanism is uniquely initiated in plants by DEMETER (DME)-class DNA glycosylases. Arabidopsis DME family glycosylases contain a conserved helix-hairpin-helix domain present in both prokaryotic and eukaryotic DNA glycosylases as well as two domains A and B of unknown function that are unique to this family. Here, we employed a mutagenesis approach to screen for DME residues critical for DNA glycosylase activity. This analysis revealed that amino acids clustered in all three domains, but not in the intervening variable regions, are required for in vitro 5-methylcytosine excision activity. Amino acids in domain A were found to be required for nonspecific DNA binding, a prerequisite for 5-methylcytosine excision. In addition, mutational analysis confirmed the importance of the iron-sulfur cluster motif to base excision activity. Thus, the DME DNA glycosylase has a unique structure composed of three essential domains that all function in 5-methylcytosine excision.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.