ArticleProceedings of the National Academy of Sciences of the United States of America2010
Nucleosome eviction and activated transcription require p300 acetylation of histone H3 lysine 14.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 70 citations in OpenAlex.
- Epigenetic landscape of synthetic human centromere core regions: CENP-A assembly and euchromatic modifications interdependently antagonize heterochromatin accumulation.Nucleic acids research · 2026Article
- Conformations of Linker Histone H1 Bound to Nucleosome Arrays.Journal of molecular biology · 2026Article
- Epi-nutrients for cancer prevention: Molecular mechanisms and emerging insights.Cell biology and toxicology · 2025Review
- Identification of molecular determinants of gene-specific bursting patterns by high-throughput imaging screens.Molecular cell · 2025Article
- Mechanisms and cross-talk of regulated cell death and their epigenetic modifications in tumor progression.Molecular cancer · 2024Review
- Identification of molecular determinants of gene-specific bursting patterns by high-throughput imaging screens.bioRxiv : the preprint server for biology · 2024Article
- Histone H3 Acetylation Is Involved in Retinoid Acid-Induced Neural Differentiation through Increasing Mitochondrial Function.Biomedicines · 2023Article
- NASP maintains histone H3-H4 homeostasis through two distinct H3 binding modes.Nucleic acids research · 2022Article
- Breakers and amplifiers in chromatin circuitry: acetylation and ubiquitination control the heterochromatin machinery.Current opinion in structural biology · 2021Review
- BRG1 knockdown inhibits proliferation through multiple cellular pathways in prostate cancer.Clinical epigenetics · 2021Article
- Histone Acetylation Domains Are Differentially Induced during Development of Heart Failure in Dahl Salt-Sensitive Rats.International journal of molecular sciences · 2021Article
- NAP1-Related Protein 1 (NRP1) has multiple interaction modes for chaperoning histones H2A-H2B.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- An embryonic stem cell-specific heterochromatin state promotes core histone exchange in the absence of DNA accessibility.Nature communications · 2020Article
- Heart failure drug proscillaridin A targets MYC overexpressing leukemia through global loss of lysine acetylation.Journal of experimental & clinical cancer research : CR · 2019Article
- De novo formation and epigenetic maintenance of centromere chromatin.Current opinion in cell biology · 2019Review
- Acetylation & Co: an expanding repertoire of histone acylations regulates chromatin and transcription.Essays in biochemistry · 2019Review
- Maternal hyperglycemia disturbs neocortical neurogenesis via epigenetic regulation in C57BL/6J mice.Cell death & disease · 2019Article
- The histone chaperone NAP1L3 is required for haematopoietic stem cell maintenance and differentiation.Scientific reports · 2018Article
- The Inherent Asymmetry of DNA Replication.Annual review of cell and developmental biology · 2017Review
- CRISPR-Mediated Epigenome Editing.The Yale journal of biology and medicine · 2016Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Histone posttranslational modifications and chromatin dynamics are inextricably linked to eukaryotic gene expression. Among the many modifications that have been characterized, histone tail acetylation is most strongly correlated with transcriptional activation. In Metazoa, promoters of transcriptionally active genes are generally devoid of physically repressive nucleosomes, consistent with the contemporaneous binding of the large RNA polymerase II transcription machinery. The histone acetyltransferase p300 is also detected at active gene promoters, flanked by regions of histone hyperacetylation. Although the correlation between histone tail acetylation and gene activation is firmly established, the mechanisms by which acetylation facilitates this fundamental biological process remain poorly understood. To explore the role of acetylation in nucleosome dynamics, we utilized an immobilized template carrying a natural promoter reconstituted with various combinations of wild-type and mutant histones. We find that the histone H3 N-terminal tail is indispensable for activator, p300, and acetyl-CoA-dependent nucleosome eviction mediated by the histone chaperone Nap1. Significantly, we identify H3 lysine 14 as the essential p300 acetylation substrate required for dissociation of the histone octamer from the promoter DNA. Together, a total of 11 unique mutant octamer sets corroborated these observations and revealed a striking correlation between nucleosome eviction and strong activator and acetyl-CoA-dependent transcriptional activation. These novel findings uncover an exclusive role for H3 lysine 14 acetylation in facilitating the ATP-independent and transcription-independent disassembly of promoter nucleosomes by Nap1. Furthermore, these studies directly couple nucleosome disassembly with strong, activator-dependent transcription.
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