ArticleThe Journal of biological chemistry2010
Eosinophil cysteinyl leukotriene synthesis mediated by exogenous secreted phospholipase A2 group X.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 51 citations in OpenAlex.
- Article
- Emerging Role of Phospholipase-Derived Cleavage Products in Regulating Eosinophil Activity: Focus on Lysophospholipids, Polyunsaturated Fatty Acids and Eicosanoids.International journal of molecular sciences · 2021Review
- Secreted Phospholipase AJournal of immunology (Baltimore, Md. : 1950) · 2020Article
- Function of secreted phospholipase ABiochimica et biophysica acta. Molecular and cell biology of lipids · 2019Review
- Harmful and protective roles of group V phospholipase ABiochimica et biophysica acta. Molecular and cell biology of lipids · 2019Review
- Secreted PLA2 group X orchestrates innate and adaptive immune responses to inhaled allergen.JCI insight · 2017Article
- PLA2G5 regulates transglutaminase activity of human IL-4-activated M2 macrophages through PGE2 generation.Journal of leukocyte biology · 2016Article
- Group X Secreted Phospholipase A2 Releases ω3 Polyunsaturated Fatty Acids, Suppresses Colitis, and Promotes Sperm Fertility.The Journal of biological chemistry · 2016Article
- Endogenous secreted phospholipase A2 group X regulates cysteinyl leukotrienes synthesis by human eosinophils.The Journal of allergy and clinical immunology · 2016Article
- The Roles of the Secreted Phospholipase AAdvances in immunology · 2016Review
- A new era of secreted phospholipase A₂.Journal of lipid research · 2015Article
- The role of prostaglandins in allergic lung inflammation and asthma.Expert review of respiratory medicine · 2015Review
- The step further to understand the role of cytosolic phospholipase A2 alpha and group X secretory phospholipase A2 in allergic inflammation: pilot study.BioMed research international · 2014Article
- Role of cells and mediators in exercise-induced bronchoconstriction.Immunology and allergy clinics of North America · 2013Review
- Regulation and function of epithelial secreted phospholipase A2 group X in asthma.American journal of respiratory and critical care medicine · 2013Article
- The involvement of phospholipases A2 in asthma and chronic obstructive pulmonary disease.Mediators of inflammation · 2013Review
- Epithelial regulation of eicosanoid production in asthma.Pulmonary pharmacology & therapeutics · 2012Review
- New insights into pathogenesis of exercise-induced bronchoconstriction.Current opinion in allergy and clinical immunology · 2012Review
- Transcriptional Changes of Blood Eosinophils After Methacholine Inhalation Challenge in Asthmatics.Genomics insights · 2012Article
- Group X secreted phospholipase A2 proenzyme is matured by a furin-like proprotein convertase and releases arachidonic acid inside of human HEK293 cells.The Journal of biological chemistry · 2011Article
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Authors and funding
8 authors at 1 institution in 1 country.
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Abstract
Secreted phospholipase A(2) group X (sPLA(2)-X) has recently been identified in the airways of patients with asthma and may participate in cysteinyl leukotriene (CysLT; C(4), D(4), and E(4)) synthesis. We examined CysLT synthesis and arachidonic acid (AA) and lysophospholipid release by eosinophils mediated by recombinant human sPLA(2)-X. We found that recombinant sPLA(2)-X caused marked AA release and a rapid onset of CysLT synthesis in human eosinophils that was blocked by a selective sPLA(2)-X inhibitor. Exogenous sPLA(2)-X released lysophospholipid species that arise from phospholipids enriched in AA in eosinophils, including phosphatidylcholine, phosphatidylinositol, and phosphatidylethanolamine as well as plasmenyl phosphatidylcholine and phosphatidylethanolamine. CysLT synthesis mediated by sPLA(2)-X but not AA release could be suppressed by inhibition of cPLA(2)α. Exogenous sPLA(2)-X initiated Ser(505) phosphorylation of cPLA(2)α, an intracellular Ca(2+) flux, and translocation of cPLA(2)α and 5-lipoxygenase in eosinophils. Synthesis of CysLTs in response to sPLA(2)-X or lysophosphatidylcholine was inhibited by p38 or JNK inhibitors but not by a MEK 1/2 inhibitor. A further increase in CysLT synthesis was induced by the addition of sPLA(2)-X to eosinophils under conditions of N-formyl-methionyl-leucyl-phenylalanine-mediated cPLA(2)α activation. These results indicate that sPLA(2)-X participates in AA and lysophospholipid release, resulting in CysLT synthesis in eosinophils through a mechanism involving p38 and JNK MAPK, cPLA(2)α, and 5-lipoxygenase activation and resulting in the amplification of CysLT synthesis during cPLA(2)α activation. Transactivation of eosinophils by sPLA(2)-X may be an important mechanism leading to CysLT formation in the airways of patients with asthma.
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