ArticleThe Journal of biological chemistry2010
Simultaneous observation of tail and head movements of myosin V during processive motion.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 19 citations in OpenAlex.
- Myosin-5 varies its step length to carry cargo straight along the irregular F-actin track.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Myosin-5 varies its steps along the irregular F-actin track.bioRxiv : the preprint server for biology · 2023Article
- Evidence for S2 flexibility by direct visualization of quantum dot-labeled myosin heads and rods within smooth muscle myosin filaments moving on actin in vitro.The Journal of general physiology · 2021Article
- Myosin Va transport of liposomes in three-dimensional actin networks is modulated by actin filament density, position, and polarity.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Myosin Va molecular motors manoeuvre liposome cargo through suspended actin filament intersections in vitro.Nature communications · 2017Article
- Myosin Vc Is Specialized for Transport on a Secretory Superhighway.Current biology : CB · 2016Article
- Vascular disease-causing mutation R258C in ACTA2 disrupts actin dynamics and interaction with myosin.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Phosphorylation of myosin regulatory light chain has minimal effect on kinetics and distribution of orientations of cross bridges of rabbit skeletal muscle.American journal of physiology. Regulatory, integrative and comparative physiology · 2014Article
- More than just a cargo adapter, melanophilin prolongs and slows processive runs of myosin Va.The Journal of biological chemistry · 2013Article
- The kinetics of mechanically coupled myosins exhibit group size-dependent regimes.Biophysical journal · 2013Article
- Myosin VI has a one track mind versus myosin Va when moving on actin bundles or at an intersection.Traffic (Copenhagen, Denmark) · 2013Article
- Mesoscopic analysis of motion and conformation of cross-bridges.Biophysical reviews · 2012Review
- A branched kinetic scheme describes the mechanochemical coupling of Myosin Va processivity in response to substrate.Biophysical journal · 2012Article
- Quantum dots find their stride in single molecule tracking.Current opinion in chemical biology · 2011Review
- Myosin Va and myosin VI coordinate their steps while engaged in an in vitro tug of war during cargo transport.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Processive stepping of myosin Va (myoV) has been tracked by monitoring either the tail position (center of mass) or the position of one or both heads. Here, we combine these two approaches by attaching a quantum dot to one of the motor domains and a bead to the tail. Using laser trapping and total internal reflection microscopy, the position of one head and the tail are observed simultaneously as myoV moves processively on an actin filament bundle against the resistive load of the laser trap. The head moves one step (73 ± 10 nm) for every two steps of the tail (35 ± 9 nm). One tail step occurs concurrently with quantum dot-labeled head movement, whereas the other occurs with movement of the unlabeled head, consistent with a hand-over-hand model. Load increases the probability of the motor taking a back step. The back step is triggered by the motor taking a shorter forward step (head step, 68 ± 11 nm; tail step, 32 ± 10 nm), likely one actin monomer short of its preferred binding site. During a back step, the motor reverses its hand-over-hand motion, with the leading head detaching and reattaching to one of multiple actin sites behind the trailing head. After a back step, the motor can correct its mistake and step processively forward at resistive loads <0.7 piconewton or stall or detach at higher loads. Back stepping may provide a mechanism to ensure efficient cargo delivery even when myoV encounters obstacles within the actin cytoskeletal meshwork or when other motors are attached to the same cargo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.