ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2011
DNA bacterial load in children and adolescents with pneumococcal pneumonia and empyema.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 55 citations in OpenAlex.
- Factors Associated With Pneumonia Severity in Children: A Systematic Review.Journal of the Pediatric Infectious Diseases Society · 2018Pooled it
- Identification and Quantification of Pathogens in Pleural Fluid from Children with Complicated Pneumonia using the BioFire® FilmArray® Pneumonia Panel.Journal of the Pediatric Infectious Diseases Society · 2026Article
- Cytosolic nucleic acid sensing as driver of critical illness: mechanisms and advances in therapy.Signal transduction and targeted therapy · 2025Review
- Development of Loop-Mediated Isothermal Amplification Assay TargetingFrontiers in microbiology · 2021Article
- Cerebrospinal fluid neopterin as a biomarker of neuroinflammatory diseases.Scientific reports · 2020Observational
- Pneumonia in children admitted to the national referral hospital in Bhutan: A prospective cohort study.International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases · 2020Article
- Validation of a Loop-Mediated Isothermal Amplification Assay for Rapid Diagnosis of Invasive Pneumococcal Disease.Frontiers in cellular and infection microbiology · 2020Article
- The bacteriology in adult patients with pneumonia and parapneumonic effusions: increased yield with DNA sequencing method.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2019Article
- Association between semi-quantitative microbial load and respiratory symptoms among Thai military recruits: a prospective cohort study.BMC infectious diseases · 2018Article
- Pneumococcal Colonization Rates in Patients Admitted to a United Kingdom Hospital with Lower Respiratory Tract Infection: a Prospective Case-Control Study.Journal of clinical microbiology · 2016Article
- Estimation of the invasive disease potential of Streptococcus pneumoniae in children by the use of direct capsular typing in clinical specimens.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2015Article
- Article
- Pneumococcal colonisation density: a new marker for disease severity in HIV-infected adults with pneumonia.BMJ open · 2014Article
- Diagnostic value of serum pneumococcal DNA load during invasive pneumococcal infections.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2014Article
- Pneumococcal empyema and complicated pneumonias: global trends in incidence, prevalence, and serotype epidemiology.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2014Review
- DNA bacterial load in children with bacteremic pneumococcal community-acquired pneumonia.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2013Article
- Clinical implications of pneumococcal serotypes: invasive disease potential, clinical presentations, and antibiotic resistance.Journal of Korean medical science · 2013Review
- Article
- Serotype 3 is a common serotype causing invasive pneumococcal disease in children less than 5 years old, as identified by real-time PCR.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2012Article
- Prevalence and clonal distribution of pcpA, psrP and Pilus-1 among pediatric isolates of Streptococcus pneumoniae.PloS one · 2012Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The purpose of this investigation was to evaluate a rapid quantitative real-time polymerase chain reaction (PCR) for the direct detection and quantification of pneumococcal DNA bacterial load (DBL) in patients with pneumonia and empyema. DBL and molecular serotype detection was determined by DNA quantification of the pneumolysin (ply) gene and an additional capsular gene by real-time PCR. Plasma or pleural fluid samples from children and adolescents with confirmed pneumococcal pneumonia were analyzed. DBL was correlated with clinical parameters and outcomes. One hundred and sixty-nine patients with pneumococcal pneumonia (145 empyema) had bacterial cultures and real-time PCR assays performed. Among them, 41 (24.3%) had positive results for both, 4 (2.4%) had positive culture alone, and 124 (73.3%) had positive real-time PCR alone. The pleural fluid DBL was lower in patients with prior antibiotics (p = 0.01) and higher in patients with positive culture (p < 0.001). The pleural fluid DBL was positively correlated with serum C-reactive protein (p = 0.009), pleural fluid neutrophils (p < 0.001), and pleural fluid glucose (p < 0.001). The plasma and pleural fluid DBL were higher in patients with ≥8 days of hospital stay (p = 0.002), and the pleural fluid DBL was positively correlated with the number of hours of pleural drainage (p < 0.001). Quantification of pneumococcal DBL by real-time PCR may be helpful for the diagnosis and clinical management of pediatric patients with pneumonia and empyema.
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