ReviewPlant cell reports2011
Recombinase technology: applications and possibilities.
Review in Plant cell reports, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
60 citing papers in PubMed, 163 citations in OpenAlex.
- From Gene Knockouts to Genome Remodeling: Large DNA Fragment Deletion Technologies in Plants.Plants (Basel, Switzerland) · 2026Review
- AnMicroorganisms · 2026Article
- Directed evolution on compact landing pads yields highly efficient recombinases for large DNA integration.Nucleic acids research · 2026Article
- Synthetic crassulacean acid metabolism (SynCAM) for improving water-use efficiency in plants.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
- Development of an improved construct for spectinomycin selection in plant transformation.BMC research notes · 2025Article
- Recent advances in site-specific transgene insertion into the maize genome using recombinases and genome editing endonucleases.Frontiers in plant science · 2025Review
- Edible mushrooms as emerging biofactories for natural therapeutics and oral biopharmaceutical delivery.Frontiers in fungal biology · 2025Review
- There and turn back again: the application of phage serine integrases in eukaryotic systems.Frontiers in bioengineering and biotechnology · 2025Review
- Bacteriophage lambda site-specific recombination.Molecular microbiology · 2024Review
- Precise integration of large DNA sequences in plant genomes using PrimeRoot editors.Nature biotechnology · 2024Article
- The design of synthetic gene circuits in plants: new components, old challenges.Journal of experimental botany · 2023Review
- Unclasping potentials of genomics and gene editing in chickpea to fight climate change and global hunger threat.Frontiers in genetics · 2023Review
- Model-guided engineering of DNA sequences with predictable site-specific recombination rates.Nature communications · 2022Article
- Breaking the Restriction Barriers and Applying CRISPRi as a Gene Silencing Tool inMicroorganisms · 2022Article
- The emergence of molecular systems neuroscience.Molecular brain · 2022Review
- Site-Specific Recombination with Inverted Target Sites: A Cautionary Tale of Dicentric and Acentric Chromosomes.Genetics · 2020Article
- Phenotypic Examination ofPlants (Basel, Switzerland) · 2020Article
- A memory switch for plant synthetic biology based on the phage ϕC31 integration system.Nucleic acids research · 2020Article
- Site-specific recombinase genome engineering toolkit in maize.Plant direct · 2020Article
- Plant Biosystems Design Research Roadmap 1.0.Biodesign research · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The use of recombinases for genomic engineering is no longer a new technology. In fact, this technology has entered its third decade since the initial discovery that recombinases function in heterologous systems (Sauer in Mol Cell Biol 7(6):2087-2096, 1987). The random insertion of a transgene into a plant genome by traditional methods generates unpredictable expression patterns. This feature of transgenesis makes screening for functional lines with predictable expression labor intensive and time consuming. Furthermore, an antibiotic resistance gene is often left in the final product and the potential escape of such resistance markers into the environment and their potential consumption raises consumer concern. The use of site-specific recombination technology in plant genome manipulation has been demonstrated to effectively resolve complex transgene insertions to single copy, remove unwanted DNA, and precisely insert DNA into known genomic target sites. Recombinases have also been demonstrated capable of site-specific recombination within non-nuclear targets, such as the plastid genome of tobacco. Here, we review multiple uses of site-specific recombination and their application toward plant genomic engineering. We also provide alternative strategies for the combined use of multiple site-specific recombinase systems for genome engineering to precisely insert transgenes into a pre-determined locus, and removal of unwanted selectable marker genes.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.