ArticleJournal of bacteriology2011
A cis-encoded antisense small RNA regulated by the HP0165-HP0166 two-component system controls expression of ureB in Helicobacter pylori.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 43 citations in OpenAlex.
- Correlation versus causation:mSphere · 2026Article
- AI-2 Induces Urease Expression Through Downregulation of Orphan Response Regulator HP1021 inFrontiers in medicine · 2022Article
- Battle for Metals: Regulatory RNAs at the Front Line.Frontiers in cellular and infection microbiology · 2022Review
- Complete identity and expression of StfZ, theFrontiers in microbiology · 2022Article
- Function of Non-coding RNA inFrontiers in molecular biosciences · 2021Review
- Long-chain fatty acids alter transcription ofPeerJ · 2021Article
- Riboregulation in the Major Gastric PathogenFrontiers in microbiology · 2021Review
- Bioinformatics analysis of small RNAs in Helicobacter pylori and the role of NAT‑67 under tinidazole treatment.Molecular medicine reports · 2020Article
- Activity of Streptococcus mutans VicR Is Modulated by Antisense RNA.Journal of dental research · 2018Article
- Regulatory RNAs in Virulence and Host-Microbe Interactions.Microbiology spectrum · 2018Review
- Small Antisense RNA RblR Positively Regulates RuBisCo inFrontiers in microbiology · 2017Article
- The cag-pathogenicity island encoded CncR1 sRNA oppositely modulates Helicobacter pylori motility and adhesion to host cells.Cellular and molecular life sciences : CMLS · 2016Article
- Phosphorylation-dependent and Phosphorylation-independent Regulation of Helicobacter pylori Acid Acclimation by the ArsRS Two-component System.Helicobacter · 2016Article
- Metalloregulation of Helicobacter pylori physiology and pathogenesis.Frontiers in microbiology · 2015Review
- Factors that mediate colonization of the human stomach by Helicobacter pylori.World journal of gastroenterology · 2014Review
- Helicobacter pylori 5'ureB-sRNA, a cis-encoded antisense small RNA, negatively regulates ureAB expression by transcription termination.Journal of bacteriology · 2013Article
- The complete Campylobacter jejuni transcriptome during colonization of a natural host determined by RNAseq.PloS one · 2013Article
- Transcriptome complexity and riboregulation in the human pathogen Helicobacter pylori.Frontiers in cellular and infection microbiology · 2012Review
- cis-antisense RNA, another level of gene regulation in bacteria.Microbiology and molecular biology reviews : MMBR · 2011Review
- Molecular aspects of bacterial pH sensing and homeostasis.Nature reviews. Microbiology · 2011Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Expression of urease is essential for gastric colonization by Helicobacter pylori. The increased level of urease in gastric acidity is due, in part, to acid activation of the two-component system (TCS) consisting of the membrane sensor HP0165 and its response regulator, HP0166, which regulates transcription of the seven genes of the urease gene cluster. We now find that there are two major ureAB transcripts: a 2.7-kb full-length ureAB transcript and a 1.4-kb truncated transcript lacking 3' ureB. Acidic pH (pH 4.5) results in a significant increase in transcription of ureAB, while neutral pH (pH 7.4) increases the truncated 1.4-kb transcript. Northern blot analysis with sense RNA and strand-specific oligonucleotide probes followed by 5' rapid amplification of cDNA ends detects an antisense small RNA (sRNA) encoded by the 5' ureB noncoding strand consisting of ∼290 nucleotides (5'ureB-sRNA). Deletion of HP0165 elevates the level of the truncated 1.4-kb transcript along with that of the 5'ureB-sRNA at both pH 7.4 and pH 4.5. Overexpression of 5'ureB-sRNA increases the 1.4-kb transcript, decreases the 2.7-kb transcript, and decreases urease activity. Electrophoretic mobility shift assay shows that unphosphorylated HP0166 binds specifically to the 5'ureB-sRNA promoter. The ability of the HP0165-HP0166 TCS to both increase and decrease ureB expression at low and high pHs, respectively, facilitates gastric habitation and colonization over the wide range of intragastric pHs experienced by the organism.
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Registered trials
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