ArticleJournal of bacteriology2011
In vitro studies of peptidoglycan binding and hydrolysis by the Bacillus anthracis germination-specific lytic enzyme SleB.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 50 citations in OpenAlex.
- SwsB Acts as a Muramic-δ-lactam Cyclase inBiochemistry · 2025Article
- Recent progress in proteins regulating the germination ofJournal of bacteriology · 2025Review
- Reconstituting Spore Cortex Peptidoglycan Biosynthesis Reveals a Deacetylase That Catalyzes Transamidation.Biochemistry · 2023Article
- Regulation of peptidoglycan hydrolases: localization, abundance, and activity.Current opinion in microbiology · 2023Review
- Article
- The SpoVA membrane complex is required for dipicolinic acid import during sporulation and export during germination.Genes & development · 2022Article
- SwsB and SafA Are Required for CwlJ-Dependent Spore Germination inJournal of bacteriology · 2020Article
- YpeB dimerization may be required to stabilize SleB for effective germination of Bacillus anthracis spores.BMC microbiology · 2019Article
- Article
- Lytic transglycosylases: concinnity in concision of the bacterial cell wall.Critical reviews in biochemistry and molecular biology · 2017Review
- Expression of prophage-encoded endolysins contributes to autolysis of Lactococcus lactis.Applied microbiology and biotechnology · 2017Article
- Wall Teichoic Acids Are Involved in the Medium-Induced Loss of Function of the Autolysin CD11 against Clostridium difficile.Scientific reports · 2016Article
- Bacillus thuringiensis peptidoglycan hydrolase SleB171 involved in daughter cell separation during cell division.Acta biochimica et biophysica Sinica · 2016Article
- Identification of MltG as a potential terminase for peptidoglycan polymerization in bacteria.Molecular microbiology · 2016Article
- Role of YpeB in cortex hydrolysis during germination of Bacillus anthracis spores.Journal of bacteriology · 2014Article
- Spore germination mediated by Bacillus megaterium QM B1551 SleL and YpeB.Journal of bacteriology · 2014Article
- Functional analysis of SleC from Clostridium difficile: an essential lytic transglycosylase involved in spore germination.Microbiology (Reading, England) · 2014Article
- Activity and regulation of various forms of CwlJ, SleB, and YpeB proteins in degrading cortex peptidoglycan of spores of Bacillus species in vitro and during spore germination.Journal of bacteriology · 2013Article
- Bacterial cell-wall recycling.Annals of the New York Academy of Sciences · 2013Review
- Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The Bacillus anthracis endospore loses resistance properties during germination when its cortex peptidoglycan is degraded by germination-specific lytic enzymes (GSLEs). Although this event normally employs several GSLEs for complete cortex removal, the SleB protein alone can facilitate enough cortex hydrolysis to produce vulnerable spores. As a means to better understand its enzymatic function, SleB was overexpressed, purified, and tested in vitro for depolymerization of cortex by measurement of optical density loss and the solubilization of substrate. Its ability to bind peptidoglycan was also investigated. SleB functions independently as a lytic transglycosylase on both intact and fragmented cortex. Most of the muropeptide products that SleB generates are large and are potential substrates for other GSLEs present in the spore. Study of a truncated protein revealed that SleB has two domains. The N-terminal domain is required for stable peptidoglycan binding, while the C-terminal domain is the region of peptidoglycan hydrolytic activity. The C-terminal domain also exhibits dependence on cortex containing muramic-δ-lactam in order to carry out hydrolysis. As the conditions and limitations for SleB activity are further elucidated, they will enable the development of treatments that stimulate premature germination of B. anthracis spores, greatly simplifying decontamination measures.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.