Evidence map›Paper›PMID 20971873›Full record

ArticleApplied and environmental microbiology2010

Growth and virulence properties of biofilm-forming Salmonella enterica serovar typhimurium under different acidic conditions.

Hua Xu, Hyeon-Yong Lee, Juhee Ahn

Abstract read
In one paragraph

Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Changing Patterns ofFrontiers in microbiology · 2021
    Article
  5. Rapid detection of3 Biotech · 2019
    Article
  6. Cell-Free Spent Media Obtained fromFrontiers in microbiology · 2016
    Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Hua XuMedical & Bio-Materials Research Center and Division of Biomaterials Engineering, Kangwon National University, Chucheon, Gangwon 200-701, Republic of Korea.
Hyeon-Yong Lee
Juhee Ahn

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study was designed to characterize the viability and potential virulence of bofilm-forming Salmonella enterica serovar Typhimurium under different pH levels, ranging from 5 to 7. The plate count method and real-time reverse transcription-PCR (RT-PCR) were used to evaluate the survival of S. Typhimurium grown in Trypticase soy broth (TSB) adjusted to pH 5, 6, and 7 (TSB-5, TSB-6, and TSB-7, respectively) at 37°C for 10 days. In TSB-5 and TSB-6, the numbers of viable cells estimated by using the real-time RT-PCR were greater than the culturable counts enumerated by the plate count method. Reflectance micro-Fourier transform infrared (micro-FTIR) spectroscopy was used to evaluate the biochemical changes in biofilm cells. Considerable changes in chemical components were observed in the biofilm cells grown in TSB-5 and TSB-6 when compared to the cells grown in TSB-7. The enterotoxin production and invasive ability of planktonic and biofilm S. Typhimurium cells were inferred by the relative levels of expression of stn and invA. The levels of expression of stn and invA were significantly increased in biofilm S. Typhimurium cells grown in TSB-5 (1.9-fold and 3.2-fold) and TSB-6 (2.1-fold and 22.3-fold) after 10 days of incubation. These results suggest that the biofilm-forming S. Typhimurium under different pH levels might change the virulence production and stress response mechanisms.

Indexed as

AcidsBiofilmsColony Count, MicrobialCulture MediaEnterotoxinsGene Expression ProfilingHydrogen-Ion ConcentrationMetabolomeMicrobial ViabilityReverse Transcriptase Polymerase Chain ReactionSalmonella typhimuriumSpectroscopy, Fourier Transform InfraredTemperatureTime FactorsVirulenceAcidsCulture MediaEnterotoxins

Identifiers

PMID20971873
PMCPMC3008236

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.