ArticleAmerican journal of physiology. Lung cellular and molecular physiology2011
Cold exposure reveals two populations of microtubules in pulmonary endothelia.
Article in American journal of physiology. Lung cellular and molecular physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 31 citations in OpenAlex.
- Distinct Right Ventricular Performance in Response to Acute Colchicine Treatment in Healthy and Diseased States.Function (Oxford, England) · 2025Article
- Cold-induced meiotic nondisjunction inmicroPublication biology · 2025Article
- Lung endothelium, tau, and amyloids in health and disease.Physiological reviews · 2024Review
- Microtubule destabilization with colchicine increases the work output of myocardial slices.Journal of molecular and cellular cardiology plus · 2024Article
- ErbB3 is a critical regulator of cytoskeletal dynamics in brain microvascular endothelial cells: Implications for vascular remodeling and blood brain barrier modulation.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2021Article
- Cystatin C regulates the cytotoxicity of infection-induced endothelial-derived β-amyloid.FEBS open bio · 2020Article
- Spastin mutations impair coordination between lipid droplet dispersion and reticulum.PLoS genetics · 2020Article
- Functional differences of short and long isoforms of spastin harboring missense mutation.Disease models & mechanisms · 2018Article
- Indibulin dampens microtubule dynamics and produces synergistic antiproliferative effect with vinblastine in MCF-7 cells: Implications in cancer chemotherapy.Scientific reports · 2018Article
- The β3-integrin endothelial adhesome regulates microtubule-dependent cell migration.EMBO reports · 2018Article
- A centrosomal protein STARD9 promotes microtubule stability and regulates spindle microtubule dynamics.Cell cycle (Georgetown, Tex.) · 2018Article
- Calcium depletion destabilises kinetochore fibres by the removal of CENP-F from the kinetochore.Scientific reports · 2017Article
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2017Article
- Cigarette Smoke Disrupted Lung Endothelial Barrier Integrity and Increased Susceptibility to Acute Lung Injury via Histone Deacetylase 6.American journal of respiratory cell and molecular biology · 2016Article
- Novel cis-selective and non-epimerisable C3 hydroxy azapodophyllotoxins targeting microtubules in cancer cells.European journal of medicinal chemistry · 2016Article
- Pseudomonas aeruginosa exoenzymes U and Y induce a transmissible endothelial proteinopathy.American journal of physiology. Lung cellular and molecular physiology · 2016Article
- Heterogeneity of pulmonary endothelial cyclic nucleotide response to Pseudomonas aeruginosa ExoY infection.American journal of physiology. Lung cellular and molecular physiology · 2015Article
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- MAP6-F is a temperature sensor that directly binds to and protects microtubules from cold-induced depolymerization.The Journal of biological chemistry · 2012Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Microtubules are composed of α-tubulin and β-tubulin dimers. Microtubules yield tubulin dimers when exposed to cold, which reassemble spontaneously to form microtubule fibers at 37°C. However, mammalian neurons, glial cells, and fibroblasts have cold-stable microtubules. While studying the microtubule toxicity mechanisms of the exotoxin Y from Pseudomonas aeruginosa in pulmonary microvascular endothelial cells, we observed that some endothelial microtubules were very difficult to disassemble in the cold. As a consequence, we designed studies to test the hypothesis that microvascular endothelium has a population of cold-stable microtubules. Pulmonary microvascular endothelial cells and HeLa cells (control) were grown under regular cell culture conditions, followed by exposure to an ice-cold water bath and a microtubule extraction protocol. Polymerized microtubules were detected by immunofluorescence confocal microscopy and Western blot analyses. After cold exposure, immunofluorescence revealed that the majority of HeLa cell microtubules disassembled, whereas a smaller population of endothelial cell microtubules disassembled. Immunoblot analyses showed that microvascular endothelial cells express the microtubule cold-stabilizing protein N-STOP (neuronal stable tubule-only polypeptides), and that N-STOP binds to endothelial microtubules after cold exposure, but not if microtubules are disassembled with nocodazole before cold exposure. Hence, pulmonary endothelia have a population of cold-stable microtubules.
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Registered trials
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