ArticleVirology2011
Identification of tolerated insertion sites in poliovirus non-structural proteins.
Article in Virology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 21 citations in OpenAlex.
- Scanning the horizon: deep mutational scanning approaches in virology.Journal of virology · 2026Review
- Deep mutation, insertion and deletion scanning across the Enterovirus A proteome reveals constraints shaping viral evolution.Nature microbiology · 2025Article
- Uncovering Structural Plasticity ofResearch square · 2024Article
- A Redundant Mechanism of Recruitment Underlies the Remarkable Plasticity of the Requirement of Poliovirus Replication for the Cellular ArfGEF GBF1.Journal of virology · 2019Article
- Random Insertional Mutagenesis of a Serotype 2 Dengue Virus Clone.Bio-protocol · 2018Article
- Emergency Services of Viral RNAs: Repair and Remodeling.Microbiology and molecular biology reviews : MMBR · 2018Review
- Affinity proteomics to study endogenous protein complexes: pointers, pitfalls, preferences and perspectives.BioTechniques · 2015Article
- Picornavirus--host interactions to construct viral secretory membranes.Progress in molecular biology and translational science · 2015Review
- Article
- The Golgi protein ACBD3, an interactor for poliovirus protein 3A, modulates poliovirus replication.Journal of virology · 2013Article
- High-resolution functional profiling of the norovirus genome.Journal of virology · 2012Article
- Evolution of poliovirus defective interfering particles expressing Gaussia luciferase.Journal of virology · 2012Article
- Investigating the role of viral integral membrane proteins in promoting the assembly of nepovirus and comovirus replication factories.Frontiers in plant science · 2012Article
- Expression and stability of foreign epitopes introduced into 3A nonstructural protein of foot-and-mouth disease virus.PloS one · 2012Article
- Analysis of poliovirus protein 3A interactions with viral and cellular proteins in infected cells.Journal of virology · 2011Article
- Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Insertion of nucleotide sequences encoding "tags" that can be expressed in specific viral proteins during an infection is a useful strategy for purifying viral proteins and their functional complexes from infected cells and/or for visualizing the dynamics of their subcellular location over time. To identify regions in the poliovirus polyprotein that could potentially accommodate insertion of tags, transposon-mediated insertion mutagenesis was applied to the entire nonstructural protein-coding region of the poliovirus genome, followed by selection of genomes capable of generating infectious, viable viruses. This procedure allowed us to identify at least one site in each viral nonstructural protein, except protein 2C, in which a minimum of five amino acids could be inserted. The distribution of these sites is analyzed from the perspective of their protein structural context and from the perspective of virus evolution.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.