ReviewPharmacology & therapeutics2011
Single cell transcriptomics of hypothalamic warm sensitive neurons that control core body temperature and fever response Signaling asymmetry and an extension of chemical neuroanatomy.
Review in Pharmacology & therapeutics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
46 citing papers in PubMed, 83 citations in OpenAlex.
- Heat acclimation defense against exertional heat stroke by improving the function of preoptic TRPV1 neurons.Theranostics · 2025Article
- Neuronal diversity of neuropeptide signaling, including galanin, in the mouse locus coeruleus.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- A hypothalamomedullary network for physiological responses to environmental stresses.Nature reviews. Neuroscience · 2022Review
- PEN Receptor GPR83 in Anxiety-Like Behaviors: Differential Regulation in Global vs Amygdalar Knockdown.Frontiers in neuroscience · 2021Article
- Role of the Preoptic Area in Sleep and Thermoregulation.Frontiers in neuroscience · 2021Review
- Impaired function of the suprachiasmatic nucleus rescues the loss of body temperature homeostasis caused by time-restricted feeding.Science bulletin · 2020Article
- Investigating the Effects of Brainstem Neuronal Adaptation on Cardiovascular Homeostasis.Frontiers in neuroscience · 2020Article
- Thermoregulation via Temperature-Dependent PGDNeuron · 2019Article
- Central nervous system circuits that control body temperature.Neuroscience letters · 2019Review
- The successes and future prospects of the linear antisense RNA amplification methodology.Nature protocols · 2018Article
- Cellular populations and thermosensing mechanisms of the hypothalamic thermoregulatory center.Pflugers Archiv : European journal of physiology · 2018Review
- Targeting the Recently Deorphanized Receptor GPR83 for the Treatment of Immunological, Neuroendocrine and Neuropsychiatric Disorders.Progress in molecular biology and translational science · 2018Review
- Mapping Molecular Datasets Back to the Brain Regions They are Extracted from: Remembering the Native Countries of Hypothalamic Expatriates and Refugees.Advances in neurobiology · 2018Article
- Neuropeptide and Small Transmitter Coexistence: Fundamental Studies and Relevance to Mental Illness.Frontiers in neural circuits · 2018Review
- Oligonucleotide modifications enhance probe stability for single cell transcriptome in vivo analysis (TIVA).Organic & biomolecular chemistry · 2017Article
- Insulin-like growth factor 1 receptor regulates hypothermia during calorie restriction.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Neuro-psychopharmacological perspective of Orphan receptors of Rhodopsin (class A) family of G protein-coupled receptors.Psychopharmacology · 2017Review
- The TRPM2 channel is a hypothalamic heat sensor that limits fever and can drive hypothermia.Science (New York, N.Y.) · 2016Article
- Depression-like behavior in rat: Involvement of galanin receptor subtype 1 in the ventral periaqueductal gray.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Amiloride-Insensitive Salt Taste Is Mediated by Two Populations of Type III Taste Cells with Distinct Transduction Mechanisms.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
We report on an 'unbiased' molecular characterization of individual, adult neurons, active in a central, anterior hypothalamic neuronal circuit, by establishing cDNA libraries from each individual, electrophysiologically identified warm sensitive neuron (WSN). The cDNA libraries were analyzed by Affymetrix microarray. The presence and frequency of cDNAs were confirmed and enhanced with Illumina sequencing of each single cell cDNA library. cDNAs encoding the GABA biosynthetic enzyme Gad1 and of adrenomedullin, galanin, prodynorphin, somatostatin, and tachykinin were found in the WSNs. The functional cellular and in vivo studies on dozens of the more than 500 neurotransmitters, hormone receptors and ion channels, whose cDNA was identified and sequence confirmed, suggest little or no discrepancy between the transcriptional and functional data in WSNs; whenever agonists were available for a receptor whose cDNA was identified, a functional response was found. Sequencing single neuron libraries permitted identification of rarely expressed receptors like the insulin receptor, adiponectin receptor 2 and of receptor heterodimers; information that is lost when pooling cells leads to dilution of signals and mixing signals. Despite the common electrophysiological phenotype and uniform Gad1 expression, WSN transcriptomes show heterogeneity, suggesting strong epigenetic influence on the transcriptome. Our study suggests that it is well-worth interrogating the cDNA libraries of single neurons by sequencing and chipping.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.