ArticleActa physiologica (Oxford, England)2011
Expression of acid-sensing ion channels in intestinal epithelial cells and their role in the regulation of duodenal mucosal bicarbonate secretion.
Article in Acta physiologica (Oxford, England), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 39 citations in OpenAlex.
- Acid-sensing ion channel 1a modulation of apoptosis in acidosis-related diseases: implications for therapeutic intervention.Cell death discovery · 2023Review
- Peripheral and central employment of acid-sensing ion channels during early bilaterian evolution.eLife · 2023Article
- Current opinion on the regulation of small intestinal magnesium absorption.World journal of gastroenterology · 2023Review
- Pathology and physiology of acid‑sensitive ion channels in the digestive system (Review).International journal of molecular medicine · 2022Review
- Distinct roles for twoeLife · 2022Article
- Neural signalling of gut mechanosensation in ingestive and digestive processes.Nature reviews. Neuroscience · 2022Review
- Acid-Sensing Ion Channels and Mechanosensation.International journal of molecular sciences · 2021Review
- Acid-Sensing Ion Channel-1a in Articular Chondrocytes and Synovial Fibroblasts: A Novel Therapeutic Target for Rheumatoid Arthritis.Frontiers in immunology · 2020Review
- Molecular mechanisms of caffeine-mediated intestinal epithelial ion transports.British journal of pharmacology · 2019Article
- The inhibitory role of purinergic P2Y receptor on MgThe journal of physiological sciences : JPS · 2019Article
- Helicobacter pylori infection downregulates duodenal CFTR and SLC26A6 expressions through TGFβ signaling pathway.BMC microbiology · 2018Article
- Identification and Function of Acid-sensing Ion Channels in RAW 264.7 Macrophage Cells.Current medical science · 2018Article
- Acid-sensing ion channels: dual function proteins for chemo-sensing and mechano-sensing.Journal of biomedical science · 2018Review
- Molecular mechanisms of calcium signaling in the modulation of small intestinal ion transports and bicarbonate secretion.Oncotarget · 2018Article
- CaOncotarget · 2017Review
- The roles of acid-sensing ion channel 1a and ovarian cancer G protein-coupled receptor 1 on passive Mg2+ transport across intestinal epithelium-like Caco-2 monolayers.The journal of physiological sciences : JPS · 2014Article
- Extracellular pH regulates zinc signaling via an Asp residue of the zinc-sensing receptor (ZnR/GPR39).The Journal of biological chemistry · 2012Article
- Evolutionary dynamics of carcinogenesis and why targeted therapy does not work.Nature reviews. Cancer · 2012Review
- ENaCs and ASICs as therapeutic targets.American journal of physiology. Cell physiology · 2012Review
- Article
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Authors and funding
6 authors at 2 institutions in 3 countries.
Funding
Abstract
aimsAs little is currently known about acid-sensing ion channels (ASICs) in intestinal epithelial cells, the aims of the present study were to investigate the expression and function of ASICs in intestinal epithelial cells, particularly their physiological role in the acid-stimulated duodenal mucosal bicarbonate secretion (DMBS).
methodsRT-PCR and digital Ca²(+) imaging were used to determine the expression and function of ASICs in HT29 cells and SCBN cells, intestinal epithelial crypt cell lines. The acid-stimulated DMBS was measured in C57 black mice in vivo to study the role of ASICs in this physiological process.
resultsASIC1a mRNA expression was detected in the duodenal mucosa stripped from mice and epithelial cell lines, in which cytoplasmic free Ca²(+) ([Ca²(+) ](cyt)) in response to extracellular acidosis was also increased. In Ca²(+) -containing solutions, acidosis (pH 6.0-5.0) raised [Ca²(+) ](cyt) in both HT29 cells and SCBN cells in a similar pH-dependent manner. Acidosis-induced increase in [Ca²(+) ](cyt) was markedly inhibited by amiloride (an ASICs blocker), SK&F96365 (a blocker for non-selective cation channels), or in Ca²(+) -free solutions; but was abolished by amiloride in Ca²(+) -free solutions. However, acidosis-induced increase in [Ca²(+) ](cyt) was slightly affected by U73122 (a PLC inhibitor), or nifedipine (a voltage-gated Ca²(+) channel blocker). After acidosis raised [Ca²(+) ](cyt) , stimulation of purinergic receptors with ATP further increased [Ca²(+) ](cyt) , but acidosis-induced increase in [Ca²(+) ](cyt) was not altered by suramin. Moreover, acid-stimulated murine DMBS was significantly attenuated by amiloride.
conclusionTherefore, ASICs are functionally expressed in intestinal epithelial cells, and may play a role in acid-stimulated DMBS through a Ca²(+) signalling pathway.
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