ArticleThe Journal of biological chemistry2010
Imaging of mobile long-lived nanoplatforms in the live cell plasma membrane.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 115 citations in OpenAlex.
- Immunomodulatory effect of NSAID in geriatric patients with acute infection: effects of piroxicam on chemokine/cytokine secretion patterns and levels of heat shock proteins. A double-blind randomized controlled trial. (ISRCTN58517443).Cell stress & chaperones · 2012Trial
- Probing SARS-CoV-2 membrane binding peptide via single-molecule AFM-based force spectroscopy.Nature communications · 2025Article
- Correlative single-molecule and structured illumination microscopy of fast dynamics at the plasma membrane.Nature communications · 2024Article
- Monomeric agonist peptide/MHCII complexes activate T-cells in an autonomous fashion.EMBO reports · 2023Article
- Analytical Techniques for Single-Cell Biochemical Assays of Lipids.Annual review of biomedical engineering · 2023Review
- Monte Carlo simulations for the evaluation of oligomerization data in TOCCSL experiments.Biophysical journal · 2023Article
- APP and Bace1: Differential effect of cholesterol enrichment on processing and plasma membrane mobility.iScience · 2023Article
- The plasma membrane as an adaptable fluid mosaic.Biochimica et biophysica acta. Biomembranes · 2023Review
- Strategies for the Site-Specific Decoration of DNA Origami Nanostructures with Functionally Intact Proteins.ACS nano · 2021Article
- Calcium levels in the Golgi complex regulate clustering and apical sorting of GPI-APs in polarized epithelial cells.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Cathelicidin and PMB neutralize endotoxins by multifactorial mechanisms including LPS interaction and targeting of host cell membranes.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Determination of the size of lipid rafts studied through single-molecule FRET simulations.Biophysical journal · 2021Article
- STED lithography in microfluidics for 3D thrombocyte aggregation testing.Journal of nanobiotechnology · 2021Article
- Homo- and Heteroassociations Drive Activation of ErbB3.Biophysical journal · 2019Article
- A step closer in defining glycosylphosphatidylinositol anchored proteins role in health and glycosylation disorders.Molecular genetics and metabolism reports · 2018Article
- A mixed alchemical and equilibrium dynamics to simulate heterogeneous dense fluids: Illustrations for Lennard-Jones mixtures and phospholipid membranes.The Journal of chemical physics · 2018Article
- Determination of the Membrane Environment of CD59 in Living Cells.Biomolecules · 2018Article
- Monomeric TCRs drive T cell antigen recognition.Nature immunology · 2018Article
- GPI-anchored proteins are confined in subdiffraction clusters at the apical surface of polarized epithelial cells.The Biochemical journal · 2017Article
- Mild heat induces a distinct "eustress" response in Chinese Hamster Ovary cells but does not induce heat shock protein synthesis.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
The plasma membrane has been hypothesized to contain nanoscopic lipid platforms, which are discussed in the context of "lipid rafts" or "membrane rafts." Based on biochemical and cell biological studies, rafts are believed to play a crucial role in many signaling processes. However, there is currently not much information on their size, shape, stability, surface density, composition, and heterogeneity. We present here a method that allows for the first time the direct imaging of nanoscopic long-lived platforms with raft-like properties diffusing in the live cell plasma membrane. Our method senses these platforms by their property to assemble a characteristic set of fluorescent marker proteins or lipids on a time scale of seconds. A special photobleaching protocol was used to reduce the surface density of labeled mobile platforms down to the level of well isolated diffraction-limited spots without altering the single spot brightness. The statistical distribution of probe molecules per platform was determined by single molecule brightness analysis. For demonstration, we used the consensus raft marker glycosylphosphatidylinositol-anchored monomeric GFP and the fluorescent lipid analog BODIPY-G(M1), which preferentially partitions into liquid-ordered phases. For both markers, we found cholesterol-dependent homo-association in the plasma membrane of living CHO and Jurkat T cells in the resting state, thereby demonstrating the existence of small, mobile, long-lived platforms containing these probes. We further applied the technology to address structural changes in the plasma membrane during fever-type heat shock: at elevated temperatures, the glycosylphosphatidylinositol-anchored monomeric GFP homo-association disappeared, accompanied by an increase in the expression of the small heat shock protein Hsp27.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.