ArticleNucleic acids research2011
Selection of Shine-Dalgarno sequences in plastids.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 66 citations in OpenAlex.
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Advances in promoter engineering strategies for enhanced recombinant protein expression in plants.Frontiers in plant science · 2025Review
- Deep learning-based investigation of chloroplast translation regulatory sequences.Frontiers in plant science · 2025Article
- Recent trends and advances in chloroplast engineering and transformation methods.Frontiers in plant science · 2025Review
- Harnessing the potential of chloroplast-derived expression elements for enhanced production of cellulases inPeerJ · 2025Article
- Engineering α-carboxysomes into plant chloroplasts to support autotrophic photosynthesis.Nature communications · 2023Article
- Advancing organelle genome transformation and editing for crop improvement.Plant communications · 2021Review
- Engineering Chloroplasts for High-Level Constitutive or Inducible Transgene Expression.Methods in molecular biology (Clifton, N.J.) · 2021Review
- Plastid-expressed Bacillus thuringiensis (Bt) cry3Bb confers high mortality to a leaf eating beetle in poplar.Plant cell reports · 2020Article
- Prediction and large-scale analysis of primary operons in plastids reveals unique genetic features in the evolution of chloroplasts.Nucleic acids research · 2019Article
- The plastid genome as a chassis for synthetic biology-enabled metabolic engineering: players in gene expression.Plant cell reports · 2018Review
- Chloroplast Translation: Structural and Functional Organization, Operational Control, and Regulation.The Plant cell · 2018Review
- Shine-Dalgarno Sequences Play an Essential Role in the Translation of Plastid mRNAs in Tobacco.The Plant cell · 2017Article
- Unique localization of the plastid-specific ribosomal proteins in the chloroplast ribosome small subunit provides mechanistic insights into the chloroplastic translation.Nucleic acids research · 2017Article
- The complete structure of the chloroplast 70S ribosome in complex with translation factor pY.The EMBO journal · 2017Article
- Article
- Towards engineering carboxysomes into C3 plants.The Plant journal : for cell and molecular biology · 2016Article
- Article
- Transgenic tobacco plants with improved cyanobacterial Rubisco expression but no extra assembly factors grow at near wild-type rates if provided with elevated CO2.The Plant journal : for cell and molecular biology · 2016Article
- Boosting riboswitch efficiency by RNA amplification.Nucleic acids research · 2015Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Like bacterial genes, most plastid (chloroplast) genes are arranged in operons and transcribed as polycistronic mRNAs. Plastid protein biosynthesis occurs on bacterial-type 70S ribosomes and translation initiation of many (but not all) mRNAs is mediated by Shine-Dalgarno (SD) sequences. To study the mechanisms of SD sequence recognition, we have analyzed translation initiation from mRNAs containing multiple SD sequences. Comparing translational efficiencies of identical transgenic mRNAs in Escherichia coli and plastids, we find surprising differences between the two systems. Most importantly, while internal SD sequences are efficiently recognized in E. coli, plastids exhibit a bias toward utilizing predominantly the 5'-most SD sequence. We propose that inefficient recognition of internal SD sequences provides the raison d'être for most plastid polycistronic transcripts undergoing post-transcriptional cleavage into monocistronic mRNAs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.