ArticleGeneral and comparative endocrinology2011
Dynamic glucoregulation and mammalian-like responses to metabolic and developmental disruption in zebrafish.
Article in General and comparative endocrinology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed, 122 citations in OpenAlex.
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- Advances in applied supramolecular technologies 2021-2025.Chemical Society reviews · 2025Review
- Conserved glucokinase regulation in zebrafish confirms therapeutic utility for pharmacologic modulation in diabetes.Communications biology · 2024Article
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- Characterization of the zebrafish as a model of ATP-sensitive potassium channel hyperinsulinism.BMJ open diabetes research & care · 2024Article
- Gluconeogenesis in the extraembryonic yolk syncytial layer of the zebrafish embryo.PNAS nexus · 2024Article
- Multivalent insulin receptor activation using insulin-DNA origami nanostructures.Nature nanotechnology · 2024Article
- Reversible Recognition-Based Boronic Acid Probes for Glucose Detection in Live Cells and Zebrafish.Journal of the American Chemical Society · 2023Article
- Research Progress on the Construction and Application of a Diabetic Zebrafish Model.International journal of molecular sciences · 2023Review
- Advances in Zebrafish for Diabetes Mellitus with Wound Model.Bioengineering (Basel, Switzerland) · 2023Review
- Development of a Zebrafish Larvae Model for Diabetic Heart Failure With Reduced Ejection Fraction.Korean circulation journal · 2023Article
- High glucose-induced ROS-accumulation in embryo-larval stages of zebrafish leads to mitochondria-mediated apoptosis.Apoptosis : an international journal on programmed cell death · 2022Article
- Nitrate exposure reprograms hepatic amino acid and nutrient sensing pathways prior to exercise: A metabolomic and transcriptomic investigation in zebrafishFrontiers in molecular biosciences · 2022Article
- Differential Responses of Neural Retina Progenitor Populations to Chronic Hyperglycemia.Cells · 2021Article
- In Vivo Toxicity Evaluation of Sugar AdulteratedMolecules (Basel, Switzerland) · 2021Article
- Hypoglycemic Activity of Aqueous Extract of Latex fromPharmaceuticals (Basel, Switzerland) · 2021Article
- Characteristics of the New Insulin-Resistant Zebrafish Model.Pharmaceuticals (Basel, Switzerland) · 2021Article
1 more citing papers are in PubMed but not listed here.
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Authors and funding
15 authors at 4 institutions in 1 country.
Funding
Abstract
Zebrafish embryos are emerging as models of glucose metabolism. However, patterns of endogenous glucose levels, and the role of the islet in glucoregulation, are unknown. We measured absolute glucose levels in zebrafish and mouse embryos, and demonstrate similar, dynamic glucose fluctuations in both species. Further, we show that chemical and genetic perturbations elicit mammalian-like glycemic responses in zebrafish embryos. We show that glucose is undetectable in early zebrafish and mouse embryos, but increases in parallel with pancreatic islet formation in both species. In zebrafish, increasing glucose is associated with activation of gluconeogenic phosphoenolpyruvate carboxykinase1 (pck1) transcription. Non-hepatic Pck1 protein is expressed in mouse embryos. We show using RNA in situ hybridization, that zebrafish pck1 mRNA is similarly expressed in multiple cell types prior to hepatogenesis. Further, we demonstrate that the Pck1 inhibitor 3-mercaptopicolinic acid suppresses normal glucose accumulation in early zebrafish embryos. This shows that pre- and extra-hepatic pck1 is functional, and provides glucose locally to rapidly developing tissues. To determine if the primary islet is glucoregulatory in early fish embryos, we injected pdx1-specific morpholinos into transgenic embryos expressing GFP in beta cells. Most morphant islets were hypomorphic, not a genetic, but embryos still exhibited persistent hyperglycemia. We conclude from these data that the early zebrafish islet is functional, and regulates endogenous glucose. In summary, we identify mechanisms of glucoregulation in zebrafish embryos that are conserved with embryonic and adult mammals. These observations justify use of this model in mechanistic studies of human metabolic disease.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.