ArticleEndocrinology2010
Energetic metabolism and human sperm motility: impact of CB₁ receptor activation.
Article in Endocrinology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it, 59 citations in OpenAlex.
- Cannabis and Male Fertility: A Systematic Review.The Journal of urology · 2019Pooled it
- Phytocannabinoids and Male Fertility: Implications ofPlants (Basel, Switzerland) · 2026Review
- Effects of marijuana and tobacco on male fertility and their relationship to genetic variation of mitochondrial cytochrome C oxidase genes.Scientific reports · 2025Article
- Influence of substance use on male reproductive health and offspring outcomes.Nature reviews. Urology · 2024Review
- THC and sperm: Impact on fertilization capability, pre-implantation in vitro development and epigenetic modifications.PloS one · 2024Article
- PUFAs and Their Derivatives as Emerging Players in Diagnostics and Treatment of Male Fertility Disorders.Pharmaceuticals (Basel, Switzerland) · 2023Review
- Cannabis and metformin on diabetic male Wistar rat sperm and reproductive organ parameters.Journal of diabetes and metabolic disorders · 2022Article
- Sperm Phosphoproteome: Unraveling Male Infertility.Biology · 2022Review
- Chronic exposure to delta-9-tetrahydrocannabinol impacts testicular volume and male reproductive health in rhesus macaques.Fertility and sterility · 2022Article
- TRPV1 channel in spermatozoa is a molecular target for ROS-mediated sperm dysfunction and differentially expressed in both natural and ART pregnancy failure.Frontiers in cell and developmental biology · 2022Article
- Lysophosphatidic Acid Improves Human Sperm Motility by Enhancing Glycolysis and Activating L-Type Calcium Channels.Frontiers in endocrinology · 2022Article
- Effects of marijuana on reproductive health: preconception and gestational effects.Current opinion in endocrinology, diabetes, and obesity · 2021Review
- Saturated fatty acids accelerate linear motility through mitochondrial ATP production in bull sperm.Reproductive medicine and biology · 2021Article
- Pathophysiology of Mitochondrial Dysfunction in Human Spermatozoa: Focus on Energetic Metabolism, Oxidative Stress and Apoptosis.Antioxidants (Basel, Switzerland) · 2021Review
- Cannabinoid signalling and effects of cannabis on the male reproductive system.Nature reviews. Urology · 2021Review
- Cannabinoid Receptors Signaling in the Development, Epigenetics, and Tumours of Male Germ Cells.International journal of molecular sciences · 2019Review
- Effects of Dietary Bisphenol A on the Reproductive Function of Gilthead Sea Bream (International journal of molecular sciences · 2019Article
- Activation of adenosine monophosphate-activated protein kinase (AMPK) enhances energy metabolism, motility, and fertilizing ability of cryopreserved spermatozoa in domestic cat model.Journal of assisted reproduction and genetics · 2019Article
- Gene Expression and Protein Synthesis in Mitochondria Enhance the Duration of High-Speed Linear Motility in Boar Sperm.Frontiers in physiology · 2019Article
- Probing the Origins of 1,800 MHz Radio Frequency Electromagnetic Radiation Induced Damage in Mouse Immortalized Germ Cells and SpermatozoaFrontiers in public health · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It has been reported that the endocannabinoid anandamide (AEA) exerts an adverse effect on human sperm motility, which has been ascribed to inhibition of mitochondrial activity. This seems to be at variance with evidence suggesting a major role of glycolysis in supplying ATP for sperm motility; furthermore, the role of AEA-binding receptors in mediating mitochondrial inhibition has not yet been explored. In this study, human sperm exposure to Met-AEA (methanandamide, nonhydrolyzable analog of AEA) in the micromolar range significantly decreased mitochondrial transmembrane potential (ΔΨm), similarly to rotenone, mitochondrial complex I inhibitor. The effect of Met-AEA (1 μm) was prevented by SR141716, CB(1) cannabinoid receptor antagonist, but not by SR144528, CB(2) antagonist, nor by iodoresiniferatoxin, vanilloid receptor antagonist. The effect of Met-AEA did not involve activation of caspase-9 or caspase-3 and was reverted by washing. In the presence of glucose, sperm exposure either to Met-AEA up to 1 μm or to rotenone for up to 18 h did not affect sperm motility. At higher doses Met-AEA produced a CB(1)-independent poisoning of spermatozoa, reducing their viability. Under glycolysis blockage, 1 μm Met-AEA, similarly to rotenone, dramatically abolished sperm motility, an effect that was prevented by SR1 and reverted by washing. In conclusion, CB(1) activation induced a nonapoptotic decrease of ΔΨm, the detrimental reflection on sperm motility of which could be revealed only under glycolysis blockage, unless very high doses of Met-AEA, producing CB(1)-independent sperm toxicity, were used. The effects of CB(1) activation reported here contribute to elucidate the relationship between energetic metabolism and human sperm motility.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.