ArticleThe Journal of physiology2010
Hydrogen ion dynamics in human red blood cells.
Article in The Journal of physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- Soluble adenylyl cyclase in nonmammalian sperm is directly controlled by pH, not by HCOProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Loss of NaActa physiologica (Oxford, England) · 2025Article
- Assessing the kinetics of oxygen-unloading from red cells using FlowScore, a flow-cytometric proxy of the functional quality of blood.EBioMedicine · 2025Article
- Modulation of the Oxygenation State and Intracellular pH of Erythrocytes by Inositol-Trispyrophosphate Investigated byJournal of cellular and molecular medicine · 2025Article
- Neutrophil-to-lymphocyte and platelet-to-lymphocyte ratios as mortality predictors in acute Aluminum phosphide (grain pills) poisoning: clinical insights and risk assessment.Toxicology research · 2024Article
- Article
- Unlocking the electrochemical functions of biomolecular condensates.Nature chemical biology · 2024Review
- Testing Green Tea Extract and Ammonium Salts as Stimulants of Physical Performance in a Forced Swimming Rat Experimental Model.International journal of molecular sciences · 2024Article
- Chloride Gradient Is Involved in Ammonium Influx in Human Erythrocytes.International journal of molecular sciences · 2024Article
- Carnosine regulation of intracellular pH homeostasis promotes lysosome-dependent tumor immunoevasion.Nature immunology · 2024Article
- Role of Plasma Membrane at Dielectric Relaxations and Intermembrane Interaction in Human Erythrocytes.Membranes · 2023Article
- The circulatory dynamics of human red blood cell homeostasis: Oxy-deoxy and PIEZO1-triggered changes.Biophysical journal · 2023Article
- Cell physiology and molecular mechanism of anion transport by erythrocyte band 3/AE1.American journal of physiology. Cell physiology · 2021Review
- Selective Neutral pH Inhibitor of Cathepsin B Designed Based on Cleavage Preferences at Cytosolic and Lysosomal pH Conditions.ACS chemical biology · 2021Article
- Carbon dioxide-dependent signal transduction in mammalian systems.Interface focus · 2021Review
- PIEZO1 and the mechanism of the long circulatory longevity of human red blood cells.PLoS computational biology · 2021Article
- Cathepsin B in neurodegeneration of Alzheimer's disease, traumatic brain injury, and related brain disorders.Biochimica et biophysica acta. Proteins and proteomics · 2020Review
- Single-cell OProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Autologous Blood Transfusion Enhances Exercise Performance-Strength of the Evidence and Physiological Mechanisms.Sports medicine - open · 2019Review
- The solute carrier SLC9C1 is a NaNature communications · 2018Article
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8 authors.
Funding
Abstract
Our understanding of pH regulation within red blood cells (RBCs) has been inferred mainly from indirect experiments rather than from in situ measurements of intracellular pH (pH(i)). The present work shows that carboxy-SNARF-1, a pH fluorophore, when used with confocal imaging or flow cytometry, reliably reports pH(i) in individual, human RBCs, provided intracellular fluorescence is calibrated using a 'null-point' procedure. Mean pH(i) was 7.25 in CO(2)/HCO(3)(-)-buffered medium and 7.15 in Hepes-buffered medium, and varied linearly with extracellular pH (slope of 0.77). Intrinsic (non-CO(2)/HCO(3)(-)-dependent) buffering power, estimated in the intact cell (85 mmol (l cell)(-1) (pH unit)(-1) at resting pH(i)), was somewhat higher than previous estimates from cell lysates (50-70 mmol (l cell)(-1) (pH unit)(-1)). Acute displacement of pH(i) (superfusion of weak acids/bases) triggered rapid pH(i) recovery. This was mediated via membrane Cl(-)/HCO(3)(-) exchange (the AE1 gene product), irrespective of whether recovery was from an intracellular acid or base load, and with no evident contribution from other transporters such as Na(+)/H(+) exchange. H(+)-equivalent flux through AE1 was a linear function of [H(+)](i) and reversed at resting pH(i), indicating that its activity is not allosterically regulated by pH(i), in contrast to other AE isoforms. By simultaneously monitoring pH(i) and markers of cell volume, a functional link between membrane ion transport, volume and pH(i) was demonstrated. RBC pH(i) is therefore tightly regulated via AE1 activity, but modulated during changes of cell volume. A comparable volume-pH(i) link may also be important in other cell types expressing anion exchangers. Direct measurement of pH(i) should be useful in future investigations of RBC physiology and pathology.
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