ArticlePlant cell reports2011
Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as the basis for sub-cellular proteomics.
Article in Plant cell reports, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 50 citations in OpenAlex.
- Functional characterisation of Target of Rapamycin (TOR) signalling in Physcomitrella.Plant cell reports · 2026Article
- Single-organelle DNA-sequencing of chloroplasts and mitochondria in Arabidopsis thaliana.BMC plant biology · 2026Article
- Differential prolyl hydroxylation by six Physcomitrella prolyl-4 hydroxylases.Computational and structural biotechnology journal · 2024Article
- A deeply conserved protease, acylamino acid-releasing enzyme (AARE), acts in ageing in Physcomitrella and Arabidopsis.Communications biology · 2023Article
- Proteomics Evidence of a Systemic Response to Desiccation in the Resurrection PlantInternational journal of molecular sciences · 2022Article
- Visualizing the Integrity of Chloroplast Envelope by Rhodamine and Nile Red Staining.Frontiers in plant science · 2021Article
- Tagging and catching: rapid isolation and efficient labeling of organelles using the covalent Spy-System in planta.Plant methods · 2020Article
- Article
- Chloroplast proteins involved in drought stress response in selected cultivars of common bean (3 Biotech · 2019Article
- Subcellular Phenotyping: Using Proteomics to Quantitatively Link Subcellular Leaf Protein and Organelle Distribution Analyses ofFrontiers in plant science · 2019Article
- Protein Extraction Methods Shape Much of the Extracted Proteomes.Frontiers in plant science · 2018Article
- Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing.Journal of visualized experiments : JoVE · 2017Article
- Bile Acid Sodium Symporter BASS6 Can Transport Glycolate and Is Involved in Photorespiratory Metabolism inThe Plant cell · 2017Article
- Does a voltage-sensitive outer envelope transport mechanism contributes to the chloroplast iron uptake?Planta · 2016Article
- Identification of Targets and Interaction Partners of Arginyl-tRNA Protein Transferase in the Moss Physcomitrella patens.Molecular & cellular proteomics : MCP · 2016Article
- The Mosaic Mutants of Cucumber: A Method to Produce Knock-Downs of Mitochondrial Transcripts.G3 (Bethesda, Md.) · 2015Article
- Specific pools of endogenous peptides are present in gametophore, protonema, and protoplast cells of the moss Physcomitrella patens.BMC plant biology · 2015Article
- Evolution and communication of subcellular compartments: An integrated approach.Plant signaling & behavior · 2014Article
- Quantitative analysis of the mitochondrial and plastid proteomes of the moss Physcomitrella patens reveals protein macrocompartmentation and microcompartmentation.Plant physiology · 2014Article
- Review on recent advances in the analysis of isolated organelles.Analytica chimica acta · 2012Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The moss Physcomitrella patens is increasingly being used as a model for plant systems biology studies. While genomic and transcriptomic resources are in place, tools and experimental conditions for proteomic studies need to be developed. In the present study we describe a rapid and efficient protocol for the simultaneous isolation of chloroplasts and mitochondria from moss protonema. Routinely, 60-100 μg mitochondrial and 3-5 mg chloroplast proteins, respectively, were obtained from 20 g fresh weight of green moss tissue. Using 14 plant compartment marker antibodies derived from seed plant and algal protein sequences, respectively, the evolutionary conservation of the compartment marker proteins in the moss was demonstrated and purity and intactness of the extracted organelles confirmed. This isolation protocol and these validated compartment markers may serve as basis for sub-cellular proteomics in P. patens and other mosses.
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