ArticleVirus genes2011
Comparison of the replication properties of murine and human calicivirus RNA-dependent RNA polymerases.
Article in Virus genes, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 29 citations in OpenAlex.
- Superior RdRp Function Drives the Dominance of Prevalent GI.3 Norovirus Lineages.Microorganisms · 2025Article
- Norovirus replication, host interactions and vaccine advances.Nature reviews. Microbiology · 2025Review
- What is the Potential Cause for the Predominance of GII.2[P16] Norovirus in Acute Gastroenteritis Outbreaks in China?China CDC weekly · 2022Article
- Norovirus antivirals: Where are we now?Medicinal research reviews · 2019Review
- Calicivirus RNA-Dependent RNA Polymerases: Evolution, Structure, Protein Dynamics, and Function.Frontiers in microbiology · 2019Review
- TLR7 Agonists Display Potent Antiviral Effects against Norovirus Infection via Innate Stimulation.Antimicrobial agents and chemotherapy · 2018Article
- In silico screening for human norovirus antivirals reveals a novel non-nucleoside inhibitor of the viral polymerase.Scientific reports · 2018Article
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- Recent Advances in the Discovery of Norovirus Therapeutics.Journal of medicinal chemistry · 2015Review
- Comprehensive review of human sapoviruses.Clinical microbiology reviews · 2015Review
- Nonnucleoside inhibitors of norovirus RNA polymerase: scaffolds for rational drug design.Antimicrobial agents and chemotherapy · 2014Article
- High-resolution functional profiling of the norovirus genome.Journal of virology · 2012Article
- Norovirus RNA synthesis is modulated by an interaction between the viral RNA-dependent RNA polymerase and the major capsid protein, VP1.Journal of virology · 2012Article
- Antiviral activity of nucleoside analogues against norovirus.Antiviral therapy · 2012Article
- Structural basis for specific recognition of substrates by sapovirus protease.Frontiers in microbiology · 2012Article
- VPg-primed RNA synthesis of norovirus RNA-dependent RNA polymerases by using a novel cell-based assay.Journal of virology · 2011Article
- Novel norovirus recombinants and of GII.4 sub-lineages associated with outbreaks between 2006 and 2010 in Belgium.Virology journal · 2011Article
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Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The human caliciviruses (CV), norovirus (NoV) and sapovirus (SaV), are major causes of outbreak gastroenteritis worldwide. To date, the investigation of human NoV and SaV replication cycles has been impeded as neither is culturable. Consequently, the recently discovered murine NoV (MNV) has been adopted as a surrogate replication model for the human CVs. In this study, we sought to compare the biochemical properties of the MNV RNA-dependent RNA polymerase (RdRp) with related human NoV and SaV-RdRps to address the suitability of MNV as a model for the human CVs. Three human NoV-RdRps (GII.b, GII.4 and GII.7), an MNV-RdRp and two human SaV-RdRps (GI and GII) were overexpressed in Escherichia coli, purified and their enzymatic activity and fidelity compared. Despite ~70% amino acid variation between the RdRp from the two different CV genera, the majority of the physiological characteristics of the RdRps were similar. All RdRps exhibited co-operative dimerisation and had optimal activity at 25°C, a pH range between 7 and 8, required 2-5 mM MnCl(2) and were inhibited with increasing NaCl concentrations. We observed RdRp activity at temperatures as low as 5°C and as high as 65°C. Using an in vitro fidelity assay, similar mutation rates were observed for the separate RdRps (1 × 10(-4)-1 × 10(-5)). This is the first report to compare the physiological, biochemical and mutational properties of the MNV-RdRp to those of the human CV-RdRps and it suggests that MNV may be directly applicable to the study of human NoV.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.