ArticleBritish journal of cancer2010
KRAS mutation detection and prognostic potential in sporadic colorectal cancer using high-resolution melting analysis.
Article in British journal of cancer, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Prevalence of KRAS G12C Mutation and Co-mutations and Associated Clinical Outcomes in Patients With Colorectal Cancer: A Systematic Literature Review.The oncologist · 2023Pooled it
- Prognostic and Predictive Cross-Roads of Microsatellite Instability and Immune Response to Colon Cancer.International journal of molecular sciences · 2020Review
- Prognostic Value of BRAF and KRAS Mutation in Relation to Colorectal Cancer Survival in Iranian Patients: Correlated to Microsatellite Instability.Journal of gastrointestinal cancer · 2020Article
- Formation of mammalian preribosomes proceeds from intermediate to composed state during ribosome maturation.The Journal of biological chemistry · 2019Article
- Combined assessment of the TNM stage andOncotarget · 2018Article
- Evolving notions on immune response in colorectal cancer and their implications for biomarker development.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2018Review
- Prognostic and Predictive Value of RAS Gene Mutations in Colorectal Cancer: Moving Beyond KRAS Exon 2.Drugs · 2015Review
- Comparison of genetic and epigenetic alterations of primary tumors and matched plasma samples in patients with colorectal cancer.PloS one · 2015Article
- Expression of ITGB1 predicts prognosis in colorectal cancer: a large prospective study based on tissue microarray.International journal of clinical and experimental pathology · 2015Article
- Mutation analysis of genes in the EGFR pathway in Head and Neck cancer patients: implications for anti-EGFR treatment response.BMC research notes · 2014Article
- Prognostic relevance of KRAS and BRAF mutations in Japanese patients with colorectal cancer.International journal of clinical oncology · 2013Article
- Anti-epidermal growth factor receptor therapy in head and neck squamous cell carcinoma: focus on potential molecular mechanisms of drug resistance.The oncologist · 2013Review
- Involvement of K-RAS mutations and amino acid substitutions in the survival of metastatic colorectal cancer patients.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2012Article
- KRAS mutation detection by high-resolution melting analysis significantly predicts clinical benefit of cetuximab in metastatic colorectal cancer.British journal of cancer · 2012Article
- Involvement of KRAS G12A mutation in the IL-2-independent growth of a human T-LGL leukemia cell line, PLT-2.Nagoya journal of medical science · 2012Article
- Circulating tumor-derived mutant mitochondrial DNA: a predictive biomarker of clinical prognosis in human squamous cell carcinoma.Oncotarget · 2012Article
- KRAS Testing: A Tool for the Implementation of Personalized Medicine.Genes & cancer · 2012Article
- Retrospective analysis of KRAS status in metastatic colorectal cancer patients: a single-center feasibility study.Clinical and experimental gastroenterology · 2012Article
- Ras in cancer and developmental diseases.Genes & cancer · 2011Article
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9 authors.
Funding
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Abstract
backgroundThe development of targeted therapies has created a pressing clinical need for molecular characterisation of cancers. In this retrospective study, high-resolution melting analysis (HRMA) was validated and implemented for screening of 164 colorectal cancer (CRC) patients to detect KRAS hot-spot mutations and to evaluate its prognostic value. Direct sequencing was used to confirm and characterise HRMA results.
methodsAfter establishing its sensitivity, HRMA was validated on seven cell lines and inter- and intra-variation were analysed. The prognostic value of KRAS mutations in CRC was evaluated using survival analysis.
resultsHRMA revealed abnormal melting patterns in 34.1% CRC samples. Kaplan-Meier survival curves revealed a significantly shorter overall (OS) and disease-free survival (DFS) for CRC patients harbouring a KRAS mutation. In the Cox regression analysis, only when colon and rectal cancer were analysed separately, KRAS mutation was a negative predictor for OS in patients with rectal cancer and DFS in those with stage II colon cancer.
conclusionsHRMA was found to be a valid screening method for KRAS mutation detection. The KRAS mutation came forward as a negative predictive factor for OS in patients with rectal cancer and for DFS in stage II colon cancer patients.
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