ArticleThe Plant cell2010
Clustered transcription factor genes regulate nicotine biosynthesis in tobacco.
Article in The Plant cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 110 papers.
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Who cites it
110 citing papers in PubMed, 245 citations in OpenAlex.
- Chemical Ontogeny in Plants: Developmental Dynamics of Specialized Metabolism and Implications for Ecology, Quality Control, and Bioprospecting.Plants (Basel, Switzerland) · 2026Review
- A sweet solution to nicotine biosynthesis.Nature plants · 2026Article
- Nicotine biosynthesis is completed by cryptic activating glucosylation.Nature communications · 2026Article
- Auxin-Induced Nicotine Inhibition Is Mediated by NaARF5 Through the Suppression of NaERF1-Like Expression and Interaction With NaERF1-Like in Nicotiana attenuata.Plant biotechnology journal · 2026Article
- Transcriptional Activation of NaNAC72 Suppresses Nicotine Biosynthesis in DNA Methyltransferase NaDRM2-like2 Mutants of Nicotiana attenuata.Plant biotechnology journal · 2026Article
- Ethylene represses jasmonate signaling to attenuate nicotine biosynthesis inFrontiers in plant science · 2026Article
- Complete nicotine biosynthesis reshapes the understanding of a classic alkaloid metabolic pathway.Frontiers in plant science · 2026Article
- AsAP2 transcriptionally activates ferulate 5-hydroxylase, diverting ferulic acid metabolism toward lignin biosynthesis in Angelica sinensis.Scientific reports · 2025Article
- Role of LEAFLESS, an AP2/ERF family transcription factor, in the regulation of trichome specialized metabolism.The New phytologist · 2025Article
- Biosynthesis of Camphane Volatile Terpenes inPlants (Basel, Switzerland) · 2025Article
- Article
- DNA methylation valley as a distinguishing feature occurs in root-specific expressed nicotine-related genes inFrontiers in plant science · 2025Article
- Evolutionary trajectory of transcription factors and selection of targets for metabolic engineering.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- Genetic regulation and manipulation of nicotine biosynthesis in tobacco: strategies to eliminate addictive alkaloids.Journal of experimental botany · 2024Review
- The AP2/ERF Transcription Factor PgERF120 Regulates Ginsenoside Biosynthesis in Ginseng.Biomolecules · 2024Article
- Plant disease resistance outputs regulated by AP2/ERF transcription factor family.Stress biology · 2024Review
- Comparative transcriptome analysis reveals nicotine metabolism is a critical component for enhancing stress response intensity of innate immunity system in tobacco.Frontiers in plant science · 2024Article
- Genome-wide analysis of theFrontiers in plant science · 2024Article
- An improved Nicotiana benthamiana bioproduction chassis provides novel insights into nicotine biosynthesis.The New phytologist · 2023Article
- Article
50 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tobacco (Nicotiana tabacum) synthesizes nicotine and related pyridine alkaloids in the root, and their synthesis increases upon herbivory on the leaf via a jasmonate-mediated signaling cascade. Regulatory NIC loci that positively regulate nicotine biosynthesis have been genetically identified, and their mutant alleles have been used to breed low-nicotine tobacco varieties. Here, we report that the NIC2 locus, originally called locus B, comprises clustered transcription factor genes of an ethylene response factor (ERF) subfamily; in the nic2 mutant, at least seven ERF genes are deleted altogether. Overexpression, suppression, and dominant repression experiments using transgenic tobacco roots showed both functional redundancy and divergence among the NIC2-locus ERF genes. These transcription factors recognized a GCC-box element in the promoter of a nicotine pathway gene and specifically activated all known structural genes in the pathway. The NIC2-locus ERF genes are expressed in the root and upregulated by jasmonate with kinetics that are distinct among the members. Thus, gene duplication events generated a cluster of highly homologous transcription factor genes with transcriptional and functional diversity. The NIC2-locus ERFs are close homologs of ORCA3, a jasmonate-responsive transcriptional activator of indole alkaloid biosynthesis in Catharanthus roseus, indicating that the NIC2/ORCA3 ERF subfamily was recruited independently to regulate jasmonate-inducible secondary metabolism in distinct plant lineages.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.