ArticleScience signaling2010
Single-cell analysis reveals that insulation maintains signaling specificity between two yeast MAPK pathways with common components.
Article in Science signaling, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 61 citations in OpenAlex.
- Shared and redundant proteins coordinate signal cross-talk between MAPK pathways in yeast.Molecular biology of the cell · 2024Article
- Navigating the ERK1/2 MAPK Cascade.Biomolecules · 2023Review
- Strain-dependent differences in coordination of yeast signalling networks.The FEBS journal · 2023Article
- Rate thresholds in cell signaling have functional and phenotypic consequences in non-linear time-dependent environments.Frontiers in cell and developmental biology · 2023Review
- Cdc42-Specific GTPase-Activating Protein Rga1 Squelches Crosstalk between the High-Osmolarity Glycerol (HOG) and Mating Pheromone Response MAPK Pathways.Biomolecules · 2021Article
- Ultrastructural plasma membrane asymmetries in tension and curvature promote yeast cell fusion.The Journal of cell biology · 2021Article
- Unfolded Protein Response and Scaffold Independent Pheromone MAP Kinase Signaling ControlJournal of fungi (Basel, Switzerland) · 2021Article
- Systematic Discovery of Short Linear Motifs Decodes Calcineurin Phosphatase Signaling.Molecular cell · 2020Article
- Crosstalk and spatiotemporal regulation between stress-induced MAP kinase pathways and pheromone signaling in budding yeast.Cell cycle (Georgetown, Tex.) · 2020Review
- Osmostress enhances activating phosphorylation of Hog1 MAP kinase by mono-phosphorylated Pbs2 MAP2K.The EMBO journal · 2020Article
- The adaptor protein Ste50 directly modulates yeast MAPK signaling specificity through differential connections of its RA domain.Molecular biology of the cell · 2019Article
- Heat-stress triggers MAPK crosstalk to turn on the hyperosmotic response pathway.Scientific reports · 2018Article
- Mitogen-activated protein kinase (MAPK) dynamics determine cell fate in the yeast mating response.The Journal of biological chemistry · 2017Article
- Single-cell RNA sequencing reveals intrinsic and extrinsic regulatory heterogeneity in yeast responding to stress.PLoS biology · 2017Article
- Investigating the underlying mechanism of Saccharomyces cerevisiae in response to ethanol stress employing RNA-seq analysis.World journal of microbiology & biotechnology · 2017Article
- The TORC2-Dependent Signaling Network in the Yeast Saccharomyces cerevisiae.Biomolecules · 2017Review
- Spatial and temporal signal processing and decision making by MAPK pathways.The Journal of cell biology · 2017Review
- Osmostress-induced gene expression--a model to understand how stress-activated protein kinases (SAPKs) regulate transcription.The FEBS journal · 2015Review
- Dynamic single cell measurements of kinase activity by synthetic kinase activity relocation sensors.BMC biology · 2015Article
- Genetic mapping of MAPK-mediated complex traits Across S. cerevisiae.PLoS genetics · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Eukaryotic cells use multiple mitogen-activated protein kinase (MAPK) cascades to evoke appropriate responses to external stimuli. In Saccharomyces cerevisiae, the MAPK Fus3 is activated by pheromone-binding heterotrimeric guanosine triphosphate-binding protein (G protein)-coupled receptors to promote mating, whereas the MAPK Hog1 is activated by hyperosmotic stress to elicit the high-osmolarity glycerol (HOG) response. Although these MAPK pathways share several upstream components, exposure to either pheromone or osmolyte alone triggers only the appropriate response. We used fluorescence localization- and transcription-specific reporters to assess activation of these pathways in individual cells on the minute and hour time scale, respectively. Dual activation of these two MAPK pathways occurred over a broad range of stimulant concentrations and temporal regimes in wild-type cells subjected to costimulation. Thus, signaling specificity is achieved through an "insulation" mechanism, not a "cross-inhibition" mechanism. Furthermore, we showed that there was a critical period during which Hog1 activity had to occur for proper insulation of the HOG pathway.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.