Evidence map›Paper›PMID 20959455›Full record

ArticleThe Journal of biological chemistry2010

Phosphorylated TP63 induces transcription of RPN13, leading to NOS2 protein degradation.

Yiping Huang, Edward A Ratovitski

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.8field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 10 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Yiping HuangDepartment of Dermatology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA.
Edward A Ratovitski
Johns Hopkins Medicine · US

Funding

The Role of P40 in Squamous Cell CarcinomasR01DE013561 · NIDCR · JOHNS HOPKINS UNIVERSITY · PI SIDRANSKY, DAVID · 2000 to 2009
$2.8M
NOS2-INTERACTING PROTEINS IN NOS2 REGULATIONSR01AI047224 · NIAID · JOHNS HOPKINS UNIVERSITY · PI RATOVITSKI, EDWARD A · 2000 to 2004
$1.4M
NIAID NIH HHS R01 AI047224NIAID NIH HHS R01-AI47224NIDCR NIH HHS R01 DE013561NIDCR NIH HHS R01-DE13561
6 · The paper itself

Abstract

Head and neck squamous cell carcinoma cells exposed to cisplatin display ATM-dependent phosphorylation of the most predominant TP63 isoform (ΔNp63α), leading to its activation as a transcription factor. Here, we found that the phospho-ΔNp63α protein binds to the genomic promoter of RPN13 through the TP63-responsive element. We further found that the phospho-ΔNp63α protein associates with other transcription factors (DDIT3 (also known as CHOP), NF-Y, and NF-κB), activating RPN13 gene transcription. Furthermore, cisplatin-induced and phospho-ΔNp63α-dependent RPN13 gene transcription leads to NOS2 degradation. Finally, we show that RPN13 knockdown by siRNA essentially rescues NOS2 from cisplatin-dependent inactivation. These data provide a novel mechanism for the phospho-ΔNp63α-dependent regulation of NOS2 function in cells upon cisplatin treatment, contributing to the cell death pathway of tumor cells.

Indexed as

Gene Expression Regulation, NeoplasticPromoter Regions, GeneticAntineoplastic AgentsBase SequenceCCAAT-Binding FactorCell Line, TumorCisplatinHumansIntracellular Signaling Peptides and ProteinsMembrane GlycoproteinsMolecular Sequence DataNitric Oxide Synthase Type IIPhosphorylationRNA, Small InterferingSequence Homology, Nucleic AcidTrans-ActivatorsADRM1 protein, humanAntineoplastic AgentsCCAAT-Binding FactorCisplatinDDIT3 protein, humanIntracellular Signaling Peptides and ProteinsMembrane GlycoproteinsNitric Oxide Synthase Type IINOS2 protein, humanRNA, Small InterferingTP63 protein, humanTrans-ActivatorsTranscription Factor CHOPTranscription FactorsTumor Suppressor Proteins

Identifiers

PMID20959455
PMCPMC3009868
OpenAlexW2077616606

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.