ArticleAnnals of the rheumatic diseases2010
NFκB activation and stimulation of chemokine production in normal human macrophages by the gadolinium-based magnetic resonance contrast agent Omniscan: possible role in the pathogenesis of nephrogenic systemic fibrosis.
Article in Annals of the rheumatic diseases, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 41 citations in OpenAlex.
- Induction of a type I interferon signature in normal human monocytes by gadolinium-based contrast agents: comparison of linear and macrocyclic agents.Clinical and experimental immunology · 2014Trial
- In vitro Effects of Gadoteric Acid and Iobitridol Toxicity and Platelet-Rich Plasma-Mediated Protection in Human Annulus Fibrosus and Nucleus Pulposus Cells.Journal of pain research · 2026Article
- Toxicity Mechanisms of Gadolinium and Gadolinium-Based Contrast Agents-A Review.International journal of molecular sciences · 2024Review
- In Vitro Studies Regarding the Safety of Chitosan and Hyaluronic Acid-Based Nanohydrogels Containing Contrast Agents for Magnetic Resonance Imaging.International journal of molecular sciences · 2022Article
- Gadolinium: pharmacokinetics and toxicity in humans and laboratory animals following contrast agent administration.Archives of toxicology · 2022Review
- Comparison of linear versus macrocyclic gadolinium chelates in rat skeletal muscle.Northern clinics of Istanbul · 2022Article
- Global gene expression analysis of systemic sclerosis myofibroblasts demonstrates a marked increase in the expression of multiple NBPF genes.Scientific reports · 2021Article
- Albumin-based nanoparticles as contrast medium for MRI: vascular imaging, tissue and cell interactions, and pharmacokinetics of second-generation nanoparticles.Histochemistry and cell biology · 2021Article
- Exposure of Macrophages to Low-Dose Gadolinium-Based Contrast Medium: Impact on Oxidative Stress and Cytokines Production.Contrast media & molecular imaging · 2018Article
- Centrality of bone marrow in the severity of gadolinium-based contrast-induced systemic fibrosis.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2016Article
- Gadolinium-based contrast agent toxicity: a review of known and proposed mechanisms.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2016Review
- Gadolinium-based compounds induce NLRP3-dependent IL-1β production and peritoneal inflammation.Annals of the rheumatic diseases · 2015Article
- Revisiting the risks of MRI with Gadolinium based contrast agents-review of literature and guidelines.Insights into imaging · 2015Article
- Lanthanum-Induced Gastrointestinal Histiocytosis.ACG case reports journal · 2015Article
- The significance of macrophage polarization subtypes for animal models of tissue fibrosis and human fibrotic diseases.Clinical and translational medicine · 2015Article
- Gadolinium compounds signaling through TLR4 and TLR7 in normal human macrophages: establishment of a proinflammatory phenotype and implications for the pathogenesis of nephrogenic systemic fibrosis.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- Gadolinium-induced fibrosis is counter-regulated by CCN3 in human dermal fibroblasts: a model for potential treatment of nephrogenic systemic fibrosis.Journal of cell communication and signaling · 2012Article
- Minimizing risk of nephrogenic systemic fibrosis in cardiovascular magnetic resonance.Journal of cardiovascular magnetic resonance : official journal of the Society for Cardiovascular Magnetic Resonance · 2012Review
- Stimulation of fibroblast proliferation by insoluble gadolinium salts.Biological trace element research · 2012Article
- Understanding nephrogenic systemic fibrosis.International journal of nephrology · 2012Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
objectiveNephrogenic systemic fibrosis (NSF) is a generalised fibrotic disorder occurring in certain individuals with renal insufficiency exposed to gadolinium-based contrast agents (GdBCA) for MRI. Histopathological examination of affected tissues shows increased numbers of activated macrophages. To elucidate the mechanisms responsible for macrophage activation, the effects of the GdBCA Omniscan on normal human macrophage global gene expression, chemokine production and nuclear factor κB (NFκB) activation was examined.
methodsNormal human monocyte-derived macrophages were incubated with Omniscan (50 mM) and their gene expression analysed by microarrays and real-time PCR. Macrophage chemokine production was assayed by multiplex ELISA. NFκB activation was assessed by NFκB nuclear localisation and quantitation of intracellular levels of inducible nitric oxide synthase (iNOS) protein. A specific cell-permeable NFκB peptide inhibitor was used to abrogate NFκB stimulation of chemokine and iNOS protein levels. CCL8/MCP-2 in affected skin of patients with NSF was examined by indirect immunofluorescence.
resultsOmniscan caused a profound change in the transcriptome of differentiated human normal macrophages in vitro, including a large increase in the expression of genes encoding CC and CXC chemokines. It induced rapid nuclear localisation of NFκB and stimulation of iNOS protein levels and chemokine production which were blocked by an NFκB inhibitory peptide. CCL8/MCP-2, the most upregulated chemokine following in vitro macrophage exposure to Omniscan, was strongly increased in NSF-affected skin.
conclusionThe GdBCA Omniscan induces potent stimulation of macrophage gene expression, NFκB activation and increased NFκB-mediated production of CC and CXC chemokines and iNOS. These alterations may play a crucial role in the pathogenesis of NSF.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.