ArticleBiophysical journal2010
Electrostatic interactions and binding orientation of HIV-1 matrix studied by neutron reflectivity.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 53 citations in OpenAlex.
- Structural molecular details of the endocytic adaptor protein CALM upon binding with phosphatidylinositol 4,5-bisphosphate-containing model membranes.Communications chemistry · 2025Article
- Probing the interactions of the HIV-1 matrix protein-derived polybasic region with lipid bilayers: insights from AFM imaging and force spectroscopy.European biophysics journal : EBJ · 2024Article
- Investigating the Orientation of an Interfacially Adsorbed Monoclonal Antibody and Its Fragments Using Neutron Reflection.Molecular pharmaceutics · 2023Article
- Uncovering a membrane-distal conformation of KRAS available to recruit RAF to the plasma membrane.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Membrane surface recognition by the ASAP1 PH domain and consequences for interactions with the small GTPase Arf1.Science advances · 2020Article
- Sensitivity of peripheral membrane proteins to the membrane context: A case study of phosphatidylserine and the TIM proteins.Biochimica et biophysica acta. Biomembranes · 2018Review
- The high-intensity reflectometer of the Jülich Centre for Neutron Science: MARIA.Journal of applied crystallography · 2018Article
- Cholesterol Promotes Protein Binding by Affecting Membrane Electrostatics and Solvation Properties.Biophysical journal · 2017Article
- Structural characterization of membrane-bound human immunodeficiency virus-1 Gag matrix with neutron reflectometry.Biointerphases · 2017Article
- Structural features and lipid binding domain of tubulin on biomimetic mitochondrial membranes.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- HIV-1 matrix-31 membrane binding peptide interacts differently with membranes containing PS vs. PI(4,5)PBiochimica et biophysica acta · 2016Article
- Membrane Binding of HIV-1 Matrix Protein: Dependence on Bilayer Composition and Protein Lipidation.Journal of virology · 2016Article
- Article
- Membrane association of the PTEN tumor suppressor: neutron scattering and MD simulations reveal the structure of protein-membrane complexes.Methods (San Diego, Calif.) · 2015Review
- The HIV-1 nucleocapsid protein recruits negatively charged lipids to ensure its optimal binding to lipid membranes.Journal of virology · 2015Article
- Structural features of membrane-bound glucocerebrosidase and α-synuclein probed by neutron reflectometry and fluorescence spectroscopy.The Journal of biological chemistry · 2015Article
- Zooming in on disordered systems: neutron reflection studies of proteins associated with fluid membranes.Biochimica et biophysica acta · 2014Review
- Myristoylation restricts orientation of the GRASP domain on membranes and promotes membrane tethering.The Journal of biological chemistry · 2014Article
- Coarse-grained simulations of the HIV-1 matrix protein anchoring: revisiting its assembly on membrane domains.Biophysical journal · 2014Article
- Conformational transition of membrane-associated terminally acylated HIV-1 Nef.Structure (London, England : 1993) · 2013Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
Abstract
The N-terminal matrix (MA) domain of the HIV-1 Gag protein is responsible for binding to the plasma membrane of host cells during viral assembly. The putative membrane-binding interface of MA was previously mapped by means of mutagenesis and analysis of its trimeric crystal structure. However, the orientation of MA on membranes has not been directly determined by experimental measurements. We present neutron reflectivity measurements that resolve the one-dimensional scattering length density profile of MA bound to a biomimetic of the native viral membrane. A molecular refinement procedure was developed using atomic structures of MA to determine the orientation of the protein on the membrane. The orientation defines a lipid-binding interface consistent with previous mutagenesis results. The MA protein maintains this orientation without the presence of a myristate group, driven only by electrostatic interactions. Furthermore, MA is found to penetrate the membrane headgroup region peripherally such that only the side chains of specific Lys and Arg residues interact with the surface. The results suggest that electrostatic interactions are sufficient to favorably orient MA on viral membrane mimics. The spatial determination of the membrane-bound protein demonstrates the ability of neutron reflectivity to discern orientation and penetration under physiologically relevant conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.