ArticleBiophysical journal2010
Spatial distribution of the state of water in frozen mammalian cells.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 57 citations in OpenAlex.
- Raman microscopy of cryofixed biological specimens for high-resolution and high-sensitivity chemical imaging.Science advances · 2024Article
- New insight into the mechanism by which antifreeze peptides regulate the physiological function of Streptococcus thermophilus subjected to freezing stress.Journal of advanced research · 2023Article
- Impact of drying and cooling rate on the survival of the desiccation-sensitive wheat pollen.Plant cell reports · 2022Article
- Lipid phase transitions in cat oocytes supplemented with deuterated fatty acids.Biophysical journal · 2021Article
- Non-Uniform Distribution of Cryoprotecting Agents in Rice Culture Cells Measured by CARS Microscopy.Plants (Basel, Switzerland) · 2021Article
- Flash Brillouin Scattering: A Confocal Technique for Measuring Glass Transitions at High Scan Rates.ACS photonics · 2021Article
- Raman Cryomicroscopic Imaging and Sample Holder for Spectroscopic Subzero Temperature Measurements.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Interfacial Interactions of Sucrose during Cryopreservation Detected by Raman Spectroscopy.Langmuir : the ACS journal of surfaces and colloids · 2019Article
- Effect of glycerol on photobleaching of cytochrome Raman lines in frozen yeast cells.European biophysics journal : EBJ · 2018Article
- Lipid Droplet Phase Transition in Freezing Cat Embryos and Oocytes Probed by Raman Spectroscopy.Biophysical journal · 2018Article
- Freezing Responses in DMSO-Based Cryopreservation of Human iPS Cells: Aggregates Versus Single Cells.Tissue engineering. Part C, Methods · 2018Article
- AFM Monitoring the Influence of Selected Cryoprotectants on Regeneration of Cryopreserved Cells Mechanical Properties.Frontiers in physiology · 2018Article
- Characterizing Intracellular Ice Formation of Lymphoblasts Using Low-Temperature Raman Spectroscopy.Biophysical journal · 2017Article
- Article
- Combinations of Osmolytes, Including Monosaccharides, Disaccharides, and Sugar Alcohols Act in Concert During Cryopreservation to Improve Mesenchymal Stromal Cell Survival.Tissue engineering. Part C, Methods · 2016Article
- Adaptive responses of cell hydration to a low temperature arrest.The Journal of physiology · 2016Article
- Channelling frozen cells to survival after thawing: opening the door to cryo-physiology.The Journal of physiology · 2016Article
- A critical analysis of cancer biobank practices in relation to biospecimen quality.Biophysical reviews · 2015Review
- Redox State of Cytochromes in Frozen Yeast Cells Probed by Resonance Raman Spectroscopy.Biophysical journal · 2015Article
- A Raman microspectroscopy study of water and trehalose in spin-dried cells.Biophysical journal · 2014Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe direct determination of the state of intracellular water, measurement of the intercellular concentration of a cryoprotectant agent (dimethylsulfoxide), and the distribution of organic material in frozen mammalian cells. Confocal Raman microspectroscopy was utilized at cryogenic temperatures with single live cells to conduct high spatial resolution measurements (350 × 350 × 700 nm), which yielded two, we believe, novel observations: 1), intracellular ice formation during fast cooling (50°C/min) causes more pronounced intracellular dehydration than slow cooling (1°C/min); and 2), intracellular dimethylsulfoxide concentration is lower (by as much as 50%) during fast cooling, decreasing the propensity for intracellular vitrification. These observations have a very significant impact for developing successful biopreservation protocols for cells used for therapeutic purposes and for cellular biofluids.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.