ArticleBiophysical journal2010
Variability in G-protein-coupled signaling studied with microfluidic devices.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 28 citations in OpenAlex.
- Individual yeast cells signal at different levels but each with good precision.Royal Society open science · 2025Article
- Coordination of MAPK and p53 dynamics in the cellular responses to DNA damage and oxidative stress.Molecular systems biology · 2022Article
- High capacity in G protein-coupled receptor signaling.Nature communications · 2018Article
- Integrating Immunology and Microfluidics for Single Immune Cell Analysis.Frontiers in immunology · 2018Review
- Fundamental trade-offs between information flow in single cells and cellular populations.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Distinct cellular states determine calcium signaling response.Molecular systems biology · 2016Article
- High-Content Quantification of Single-Cell Immune Dynamics.Cell reports · 2016Article
- Efficient entry of cell-penetrating peptide nona-arginine into adherent cells involves a transient increase in intracellular calcium.The Biochemical journal · 2015Article
- A G-protein subunit translocation embedded network motif underlies GPCR regulation of calcium oscillations.Biophysical journal · 2014Article
- Microfluidic interrogation and mathematical modeling of multi-regime calcium signaling dynamics.Integrative biology : quantitative biosciences from nano to macro · 2013Article
- Recent advances in single-cell studies of gene regulation.Current opinion in biotechnology · 2012Review
- Information transduction capacity of noisy biochemical signaling networks.Science (New York, N.Y.) · 2011Article
- The structure of dynamic GPCR signaling networks.Wiley interdisciplinary reviews. Systems biology and medicineReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Different cells, even those that are genetically identical, can respond differently to identical stimuli, but the precise source of this variability remains obscure. To study this problem, we built a microfluidic experimental system which can track responses of individual cells across multiple stimulations. We used this system to determine that amplitude variation in G-protein-activated calcium release in RAW264.7 macrophages is generally extrinsic, i.e., they arise from long-lived variations between cells and not from stochastic activation of signaling components. In the case of responses linked to P2Y family purine receptors, we estimate that approximately one-third of the observed variability in calcium release is receptor-specific. We further demonstrate that the signaling apparatus downstream of P2Y6 receptor activation is moderately saturable. These observations will be useful in constructing and constraining single-cell models of G protein-coupled calcium dynamics.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.