ArticlePLoS biology2010
The DNA/RNA-dependent RNA polymerase QDE-1 generates aberrant RNA and dsRNA for RNAi in a process requiring replication protein A and a DNA helicase.
Article in PLoS biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 85 citations in OpenAlex.
- Tandem repeat-induced sexual silencing: A Rid-dependent RNAi mechanism for fungal genome defense via translational repression.Science advances · 2025Article
- RNA damage and its implications in genome stability.DNA repair · 2025Review
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- Functions of RNAi Pathways in Ribosomal RNA Regulation.Non-coding RNA · 2024Review
- How RNA impacts DNA repair.DNA repair · 2023Review
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- RNA-Based Control of Fungal Pathogens in Plants.International journal of molecular sciences · 2023Review
- Structural insights into the dual activities of the two-barrel RNA polymerase QDE-1.Nucleic acids research · 2022Article
- Essential role of γ-clade RNA-dependent RNA polymerases in rice development and yield-related traits is linked to their atypical polymerase activities regulating specific genomic regions.The New phytologist · 2021Article
- Article
- RNA-induced initiation of transcriptional silencing (RITS) complex structure and function.RNA biology · 2019Review
- Sex-Based Mhrt Methylation Chromatinizes MeCP2 in the Heart.iScience · 2019Article
- A Non-Dicer RNase III and Four Other Novel Factors Required for RNAi-Mediated Transposon Suppression in the Human Pathogenic YeastG3 (Bethesda, Md.) · 2019Article
- From "Cellular" RNA to "Smart" RNA: Multiple Roles of RNA in Genome Stability and Beyond.Chemical reviews · 2018Review
- Drosha drives the formation of DNA:RNA hybrids around DNA break sites to facilitate DNA repair.Nature communications · 2018Article
- DNA Replication Is Required for Circadian Clock Function by Regulating Rhythmic Nucleosome Composition.Molecular cell · 2017Article
- The emerging role of RNAs in DNA damage repair.Cell death and differentiation · 2017Review
- Nuclear Noncoding RNAs and Genome Stability.Molecular cell · 2016Review
- Functional Evolution in Orthologous Cell-encoded RNA-dependent RNA Polymerases.The Journal of biological chemistry · 2016Article
- Gene Network Polymorphism Illuminates Loss and Retention of Novel RNAi Silencing Components in the Cryptococcus Pathogenic Species Complex.PLoS genetics · 2016Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
The production of aberrant RNA (aRNA) is the initial step in several RNAi pathways. How aRNA is produced and specifically recognized by RNA-dependent RNA polymerases (RdRPs) to generate double-stranded RNA (dsRNA) is not clear. We previously showed that in the filamentous fungus Neurospora, the RdRP QDE-1 is required for rDNA-specific aRNA production, suggesting that QDE-1 may be important in aRNA synthesis. Here we show that a recombinant QDE-1 is both an RdRP and a DNA-dependent RNA polymerase (DdRP). Its DdRP activity is much more robust than the RdRP activity and occurs on ssDNA but not dsDNA templates. We further show that Replication Protein A (RPA), a single-stranded DNA-binding complex that interacts with QDE-1, is essential for aRNA production and gene silencing. In vitro reconstitution assays demonstrate that QDE-1 can produce dsRNA from ssDNA, a process that is strongly promoted by RPA. Furthermore, the interaction between QDE-1 and RPA requires the RecQ DNA helicase QDE-3, a homolog of the human Werner/Bloom Syndrome proteins. Together, these results suggest a novel small RNA biogenesis pathway in Neurospora and a new mechanism for the production of aRNA and dsRNA in RNAi pathways.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.