ArticlePloS one2010
RNAi experiments in D. melanogaster: solutions to the overlooked problem of off-targets shared by independent dsRNAs.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed.
- Defining an optimal control for RNAi experiments with adult Schistosoma mansoni.Scientific reports · 2023Article
- miRNA: A Promising Therapeutic Target in Cancer.International journal of molecular sciences · 2022Review
- NEMix: single-cell nested effects models for probabilistic pathway stimulation.PLoS computational biology · 2015Article
- RNAi screening in Drosophila cells and in vivo.Methods (San Diego, Calif.) · 2014Review
- miRNA-Based Therapeutic Strategies.Current anesthesiology reports · 2013Article
- A new in vivo model of pantothenate kinase-associated neurodegeneration reveals a surprising role for transcriptional regulation in pathogenesis.Frontiers in cellular neuroscience · 2013Article
- Article
- RNAi screening: new approaches, understandings, and organisms.Wiley interdisciplinary reviews. RNAReview
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRNAi technology is widely used to downregulate specific gene products. Investigating the phenotype induced by downregulation of gene products provides essential information about the function of the specific gene of interest. When RNAi is applied in Drosophila melanogaster or Caenorhabditis elegans, often large dsRNAs are used. One of the drawbacks of RNAi technology is that unwanted gene products with sequence similarity to the gene of interest can be down regulated too. To verify the outcome of an RNAi experiment and to avoid these unwanted off-target effects, an additional non-overlapping dsRNA can be used to down-regulate the same gene. However it has never been tested whether this approach is sufficient to reduce the risk of off-targets. METHODOLOGY: We created a novel tool to analyse the occurrence of off-target effects in Drosophila and we analyzed 99 randomly chosen genes. PRINCIPAL
findingsHere we show that nearly all genes contain non-overlapping internal sequences that do show overlap in a common off-target gene.
conclusionBased on our in silico findings, off-target effects should not be ignored and our presented on-line tool enables the identification of two RNA interference constructs, free of overlapping off-targets, from any gene of interest.
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Registered trials
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