ArticlePloS one2010
Temporal gene expression profiling during rat femoral marrow ablation-induced intramembranous bone regeneration.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 52 citations in OpenAlex.
- Oxygen‑sensing histone demethylase KDM6A modulates chondrocyte‑to‑osteoblast transdifferentiation by activating the Wnt/β‑catenin pathway.International journal of molecular medicine · 2026Article
- Improved Methodology for Studying Postnatal Osteogenesis via Intramembranous Ossification in a Murine Bone Marrow Injury Model.Journal of visualized experiments : JoVE · 2025Article
- Improved Methodology for Studying Postnatal Osteogenesis via Intramembranous Ossification in a Murine Bone Marrow Injury Model.bioRxiv : the preprint server for biology · 2024Article
- Temporal gene expression profiling during early-stage traumatic temporomandibular joint bony ankylosis in a sheep model.BMC oral health · 2024Article
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- Differential bone remodeling mechanism in hindlimb unloaded and hibernating Daurian ground squirrels: a comparison between artificial and natural disuse within the same species.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2023Article
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- Activation of canonical Wnt signaling accelerates intramembranous bone regeneration in male mice.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2022Article
- Effects of sclerostin antibody on bone healing.World journal of orthopedics · 2021Review
- Differential bone remodeling mechanism in hindlimb unloaded rats and hibernating Daurian ground squirrels: a comparison between artificial and natural disuse.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2021Article
- Sclerostin inhibits Wnt signaling through tandem interaction with two LRP6 ectodomains.Nature communications · 2020Article
- Integrin-specific hydrogels modulate transplanted human bone marrow-derived mesenchymal stem cell survival, engraftment, and reparative activities.Nature communications · 2020Article
- Shape-fitting collagen-PLA composite promotes osteogenic differentiation of porcine adipose stem cells.Journal of the mechanical behavior of biomedical materials · 2019Article
- Molecular pathogenesis of fracture nonunion.Journal of orthopaedic translation · 2018Review
- Strontium ranelate stimulates trabecular bone formation in a rat tibial bone defect healing process.Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA · 2017Article
- Intraoperative delivery of the Notch ligand Jagged-1 regenerates appendicular and craniofacial bone defects.NPJ Regenerative medicine · 2017Article
- Intramembranous bone regeneration and implant placement using mechanical femoral marrow ablation: rodent models.BoneKEy reports · 2016Article
- Overview of biological mechanisms and applications of three murine models of bone repair: closed fracture with intramedullary fixation, distraction osteogenesis, and marrow ablation by reaming.Current protocols in mouse biology · 2015Review
- Effects of magnetic resonance-guided high-intensity focused ultrasound ablation on bone mechanical properties and modeling.Journal of therapeutic ultrasound · 2015Article
- Toxicogenomics profiling of bone marrow from rats treated with topotecan in combination with oxaliplatin: a mechanistic strategy to inform combination toxicity.Frontiers in genetics · 2015Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Enhanced understanding of differential gene expression and biological pathways associated with distinct phases of intramembranous bone regeneration following femoral marrow ablation surgery will improve future advancements regarding osseointegration of joint replacement implants, biomaterials design, and bone tissue engineering. A rat femoral marrow ablation model was performed and genome-wide microarray data were obtained from samples at 1, 3, 5, 7, 10, 14, 28, and 56 days post-ablation, with intact bones serving as controls at Day 0. Bayesian model-based clustering produced eight distinct groups amongst 9,062 significant gene probe sets based on similar temporal expression profiles, which were further categorized into three major temporal classes of increased, variable, and decreased expression. Osteoblastic- and osteoclastic-associated genes were found to be significantly expressed within the increased expression groups. Chondrogenesis was not detected histologically. Adipogenic marker genes were found within variable/decreased expression groups, emphasizing that adipogenesis was inhibited during osteogenesis. Differential biological processes and pathways associated with each major temporal group were identified, and significantly expressed genes involved were visually represented by heat maps. It was determined that the increased expression group exclusively contains genes involved in pathways for matrix metalloproteinases (MMPs), Wnt signaling, TGF-β signaling, and inflammatory pathways. Only the variable expression group contains genes associated with glycolysis and gluconeogenesis, the notch signaling pathway, natural killer cell mediated cytotoxicity, and the B cell receptor signaling pathway. The decreased group exclusively consists of genes involved in heme biosynthesis, the p53 signaling pathway, and the hematopoietic cell lineage. Significant biological pathways and transcription factors expressed at each time point post-ablation were also identified. These data present the first temporal gene expression profiling analysis of the rat genome during intramembranous bone regeneration induced by femoral marrow ablation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.