ArticleMolecular and cellular biology2010
Identification of a male-specific RNA binding protein that regulates sex-specific splicing of Bmdsx by increasing RNA binding activity of BmPSI.
Article in Molecular and cellular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed, 87 citations in OpenAlex.
- The complete sequence of the silkworm W chromosome uncovers its rapid evolution by large-scale duplications/deletions and translocation of W-linked genes.G3 (Bethesda, Md.) · 2026Article
- Article
- Molecular mechanisms of sex determination in Lepidoptera: current status and perspectives.Insect science · 2026Review
- The Masc-PSI complex directly induces male-type doublesex splicing in silkworms.Communications biology · 2025Article
- Understanding the genetics of sex determination in insects and its relevance to genetic pest management.Insect molecular biology · 2025Review
- Single-nucleus transcriptomics of wing sexual dimorphism and scale cell specialization in sulphur butterflies.PLoS biology · 2025Article
- Prophage-encodedeLife · 2025Article
- Transcriptomic Evidence for Cell-Autonomous Sex Differentiation of the Gynandromorphic Fat Body in the Silkworm,Journal of developmental biology · 2024Article
- New Insights into theJournal of agricultural and food chemistry · 2023Review
- Recent Advances and Future Potential of Long Non-Coding RNAs in Insects.International journal of molecular sciences · 2023Review
- A Wolbachia factor for male killing in lepidopteran insects.Nature communications · 2022Article
- A database of crop pest cell lines.In vitro cellular & developmental biology. Animal · 2022Article
- Manipulating Insect Sex Determination Pathways for Genetic Pest Management: Opportunities and Challenges.Frontiers in bioengineering and biotechnology · 2022Review
- Identification and Characterization of the Masculinizing Function of theInternational journal of molecular sciences · 2021Article
- Long Non-Coding RNAs in Insects.Animals : an open access journal from MDPI · 2021Review
- Gtsf1 is essential for proper female sex determination and transposon silencing in the silkworm, Bombyx mori.PLoS genetics · 2020Article
- Transgenic and knockout analyses of Masculinizer and doublesex illuminated the unique functions of doublesex in germ cell sexual development of the silkworm, Bombyx mori.BMC developmental biology · 2020Article
- Masc-induced dosage compensation in silkworm cultured cells.FEBS open bio · 2019Article
- Alternative splicing regulation of doublesex gene by RNA-binding proteins in the silkworm Bombyx mori.RNA biology · 2019Article
- P-element Somatic Inhibitor Protein Binding a Target Sequence inInternational journal of molecular sciences · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bmdsx is a sex-determining gene in the silkworm and is alternatively spliced in males and females. CE1 is a splicing silencer element responsible for the sex-specific splicing of Bmdsx. To identify sex-specific factors implicated in the sex-specific splicing of Bmdsx, we performed RNA affinity chromatography using CE1 RNA as a ligand. We have identified BmIMP, a Bombyx homolog of IGF-II mRNA binding protein (IMP), as a male-specific factor that specifically binds to CE1. The gene encoding BmIMP is localized on the Z chromosome and is male-specifically expressed in various tissues. Antisense inhibition of BmIMP expression increased female-specific splicing of Bmdsx pre-mRNA. Coimmunoprecipitation and glutathione S-transferase (GST) pulldown analyses demonstrated that BmIMP physically interacts with BmPSI, which has been identified as a factor implicated in the sex-specific splicing of Bmdsx, through the KH domains of BmIMP. The functional consequence of this interaction was examined using RNA mobility shift analysis. BmIMP increased BmPSI-CE1 RNA binding activity by decreasing the rate of BmPSI dissociation from CE1 RNA. Truncation analysis of BmIMP suggested that the KH domains are responsible for enhancing BmPSI-CE1 RNA binding activity. These results suggest that BmIMP may enhance the male-specific splicing of Bmdsx pre-mRNA by increasing RNA binding activity of BmPSI.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.