ArticleThe Journal of biological chemistry2010
Gradient of increasing Aurora B kinase activity is required for cells to execute mitosis.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 24 citations in OpenAlex.
- AGO2 localizes to cytokinetic protrusions in a p38-dependent manner and is needed for accurate cell division.Communications biology · 2021Article
- An Aurora kinase inhibitor, AMG900, inhibits glioblastoma cell proliferation by disrupting mitotic progression.Cancer medicine · 2018Article
- Article
- Targeting hepatocarcinogenesis model in C56BL6 mice with pan-aurora kinase inhibitor Danusertib.Journal of Cancer · 2018Article
- Large tumor suppressors 1 and 2 regulate Aurora-B through phosphorylation of INCENP to ensure completion of cytokinesis.Heliyon · 2016Article
- Article
- Targeting the INCENP IN-box-Aurora B interaction to inhibit CPC activity in vivo.Open biology · 2014Article
- High-resolution temporal analysis reveals a functional timeline for the molecular regulation of cytokinesis.Developmental cell · 2014Article
- Polo-like kinase-1 triggers histone phosphorylation by Haspin in mitosis.EMBO reports · 2014Article
- Review
- Selective disruption of aurora C kinase reveals distinct functions from aurora B kinase during meiosis in mouse oocytes.PLoS genetics · 2014Article
- Basal aurora kinase B activity is sufficient for histone H3 phosphorylation in prophase.Biology open · 2013Article
- Overexpression of hnRNPC2 induces multinucleation by repression of Aurora B in hepatocellular carcinoma cells.Oncology letters · 2013Article
- A chemical biology strategy to analyze rheostat-like protein kinase-dependent regulation.Chemistry & biology · 2013Article
- A small-molecule inhibitor of Haspin alters the kinetochore functions of Aurora B.The Journal of cell biology · 2012Article
- The Renaissance or the cuckoo clock.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2011Review
- Phosphorylation at serine 331 is required for Aurora B activation.The Journal of cell biology · 2011Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
INCENP, Borealin, Survivin, and Aurora B kinase comprise the chromosomal passenger complex, an essential regulator of mitotic events. INCENP (inner centromere protein) binds and activates Aurora B through a feedback loop involving phosphorylation of a Thr-Ser-Ser (TSS) motif near the INCENP C terminus. Here, we have examined the role of the TSS motif in vertebrate cells using an DT40 INCENP(ON/OFF) conditional knock-out cell line in which mutants are expressed in the absence of wild-type INCENP. Our analysis confirms that regulated phosphorylation of the two serine residues (presumably by Aurora B) is critical for full activation of the kinase and is essential for cell viability. Cells expressing INCENP mutants bearing either phospho-null (TAA) or phospho-mimetic (TEE) mutations exhibit significant levels of Aurora B kinase activity but fail to undergo normal spindle elongation or complete cytokinesis. This work confirms previous suggestions that INCENP can act as a rheostat, with different INCENP mutants promoting differing degrees of kinase activation. Our results also reveal that mitotic progression is accompanied by a requirement for progressively higher levels of Aurora B kinase activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.