ArticleProceedings of the National Academy of Sciences of the United States of America2010
Electrical detection of pathogenic bacteria via immobilized antimicrobial peptides.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
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Who cites it
77 citing papers in PubMed.
- Dielectrophoretic Enrichment Coupled with Impedance Spectroscopy for Real-Time Bacterial Detection and Antibiotic Susceptibility Testing Using an Interdigitated Wave Electrode Array.Sensors (Basel, Switzerland) · 2026Article
- An Interpretable Multi-Objective Machine Learning Framework for In Silico Prioritization of Anti-Diagnostics (Basel, Switzerland) · 2026Article
- Advanced biosensing strategies for high-risk foodborne pathogens: a comprehensive review ofFood chemistry. Molecular sciences · 2026Review
- Terrestrial hermit crab with electronic payloads for opportunistic roaming environmental sensing.RSC advances · 2025Article
- Pathogenic Bacterial Detection Using Vertical-Capacitance Sensor Array Immobilized with the Antimicrobial Peptide Melittin.Sensors (Basel, Switzerland) · 2024Article
- Detection of pathogenic gram-negative bacteria using an antimicrobial peptides-modified bipolar electrode-electrochemiluminescence platform.Mikrochimica acta · 2024Article
- Paper-based fluorescence sensor array with functionalized carbon quantum dots for bacterial discrimination using a machine learning algorithm.Analytical and bioanalytical chemistry · 2024Article
- Synthesis and Biological Activities of Some Metal Complexes of Peptides: A Review.Biotech (Basel (Switzerland)) · 2024Review
- Recent Advances in Colorimetric Tests for the Detection of Infectious Diseases and Antimicrobial Resistance.Diagnostics (Basel, Switzerland) · 2023Review
- Point-of-Care Diagnostic Devices for Detection ofBiosensors · 2023Review
- Separation and colorimetric detection of Escherichia coli by phage tail fiber protein combined with nano-magnetic beads.Mikrochimica acta · 2023Article
- Detection of bacteria causing mastitis in cows with immunoelectric tool.Journal of clinical laboratory analysis · 2023Article
- Recent Advances on Peptide-Based Biosensors and Electronic Noses for Foodborne Pathogen Detection.Biosensors · 2023Review
- Leveraging microfluidic dielectrophoresis to distinguish compositional variations of lipopolysaccharide inFrontiers in bioengineering and biotechnology · 2023Article
- Studying Peptide-Metal Ion Complex Structures by Solution-State NMR.International journal of molecular sciences · 2022Review
- Evaluation ofMolecules (Basel, Switzerland) · 2022Article
- Open-Bandgap Graphene-Based Field-Effect Transistor Using Oligo(phenylene-ethynylene) Interfacial Chemistry.Angewandte Chemie (International ed. in English) · 2022Article
- Selective Detection ofBiosensors · 2022Article
- Microfluidics for Environmental Applications.Advances in biochemical engineering/biotechnology · 2022Review
- Atomic force spectroscopy with magainin 1 functionalized tips and biomimetic supported lipid membranes.European biophysics journal : EBJ · 2022Article
17 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The development of a robust and portable biosensor for the detection of pathogenic bacteria could impact areas ranging from water-quality monitoring to testing of pharmaceutical products for bacterial contamination. Of particular interest are detectors that combine the natural specificity of biological recognition with sensitive, label-free sensors providing electronic readout. Evolution has tailored antimicrobial peptides to exhibit broad-spectrum activity against pathogenic bacteria, while retaining a high degree of robustness. Here, we report selective and sensitive detection of infectious agents via electronic detection based on antimicrobial peptide-functionalized microcapacitive electrode arrays. The semiselective antimicrobial peptide magainin I--which occurs naturally on the skin of African clawed frogs--was immobilized on gold microelectrodes via a C-terminal cysteine residue. Significantly, exposing the sensor to various concentrations of pathogenic Escherichia coli revealed detection limits of approximately 1 bacterium/μL, a clinically useful detection range. The peptide-microcapacitive hybrid device was further able to demonstrate both Gram-selective detection as well as interbacterial strain differentiation, while maintaining recognition capabilities toward pathogenic strains of E. coli and Salmonella. Finally, we report a simulated "water-sampling" chip, consisting of a microfluidic flow cell integrated onto the hybrid sensor, which demonstrates real-time on-chip monitoring of the interaction of E. coli cells with the antimicrobial peptides. The combination of robust, evolutionarily tailored peptides with electronic read-out monitoring electrodes may open exciting avenues in both fundamental studies of the interactions of bacteria with antimicrobial peptides, as well as the practical use of these devices as portable pathogen detectors.
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