ArticleBMC genomics2010
Transcriptome analysis of parallel-evolved Escherichia coli strains under ethanol stress.
Article in BMC genomics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
78 citing papers in PubMed.
- Mechanistic Insights into the Inhibition ofMicroorganisms · 2026Article
- An antioxidant and injectable hydrogel dressing for repairing MRSA biofilm-infected diabetic wounds.Journal of nanobiotechnology · 2026Article
- Resource presentation dictates genetic and phenotypic adaptation in yeast.BMC ecology and evolution · 2025Article
- Proteomic insights into survival strategies of Escherichia coli in perchlorate-rich Martian brines.Scientific reports · 2025Article
- Modulating bacterial function utilizing A knowledge base of transcriptional regulatory modules.Nucleic acids research · 2024Article
- Discovery of new quaternized norharmane dimers as potential anti-MRSA agents.Journal of advanced research · 2024Article
- Microbial conversion of ethanol to high-value products: progress and challenges.Biotechnology for biofuels and bioproducts · 2024Review
- Membrane Damage and Metabolic Disruption as the Mechanisms of Linalool againstFoods (Basel, Switzerland) · 2024Article
- Engineering styrene biosynthesis: designing a functional trans-cinnamic acid decarboxylase in Pseudomonas.Microbial cell factories · 2024Article
- Proteomics reveals energy limitation and amino acid consumption as antibacterial mechanism of linalool againstFood chemistry: X · 2023Article
- Transcriptomic and Metabolomic Profiling Uncovers Response Mechanisms of Alicyclobacillus acidoterrestris DSM 3922Microbiology spectrum · 2023Article
- Transcriptome Analysis of Protocatechualdehyde againstFoods (Basel, Switzerland) · 2023Article
- Inference of transcriptome signatures of Escherichia coli in long-term stationary phase.Scientific reports · 2023Article
- A comprehensive review and comparison of L-tryptophan biosynthesis inFrontiers in bioengineering and biotechnology · 2023Review
- The effects of different thermal and chemical stresses on release of outer membrane vesicles (OMVs) by ClearColi™.Archives of microbiology · 2022Article
- Adaptive laboratory evolution triggers pathogen-dependent broad-spectrum antimicrobial potency in Streptomyces.Journal, genetic engineering & biotechnology · 2022Article
- Effective inactivation ofJournal of analytical science and technology · 2022Article
- Marine Actinomycetes, New Sources of Biotechnological Products.Marine drugs · 2021Review
- IDARE2-Simultaneous Visualisation of Multiomics Data in Cytoscape.Metabolites · 2021Article
- Characterizing Escherichia coli's transcriptional response to different styrene exposure modes reveals novel toxicity and tolerance insights.Journal of industrial microbiology & biotechnology · 2021Article
18 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundUnderstanding ethanol tolerance in microorganisms is important for the improvement of bioethanol production. Hence, we performed parallel-evolution experiments using Escherichia coli cells under ethanol stress to determine the phenotypic changes necessary for ethanol tolerance.
resultsAfter cultivation of 1,000 generations under 5% ethanol stress, we obtained 6 ethanol-tolerant strains that showed an approximately 2-fold increase in their specific growth rate in comparison with their ancestor. Expression analysis using microarrays revealed that common expression changes occurred during the adaptive evolution to the ethanol stress environment. Biosynthetic pathways of amino acids, including tryptophan, histidine, and branched-chain amino acids, were commonly up-regulated in the tolerant strains, suggesting that activating these pathways is involved in the development of ethanol tolerance. In support of this hypothesis, supplementation of isoleucine, tryptophan, and histidine to the culture medium increased the specific growth rate under ethanol stress. Furthermore, genes related to iron ion metabolism were commonly up-regulated in the tolerant strains, which suggests the change in intracellular redox state during adaptive evolution.
conclusionsThe common phenotypic changes in the ethanol-tolerant strains we identified could provide a fundamental basis for designing ethanol-tolerant strains for industrial purposes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.