ArticleTheScientificWorldJournal2010
Design and generation of MLPA probe sets for combined copy number and small-mutation analysis of human genes: EGFR as an example.
Article in TheScientificWorldJournal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Essential thrombocythaemia progression to the fibrotic phase is associated with a decrease in JAK2 and PDL1 levels.Annals of hematology · 2022Article
- Yield of clinically reportable genetic variants in unselected cerebral palsy by whole genome sequencing.NPJ genomic medicine · 2021Article
- Profile of Basal Cell Carcinoma Mutations and Copy Number Alterations - Focus on Gene-Associated Noncoding Variants.Frontiers in oncology · 2021Article
- Novel fluorescent-based reporter cell line engineered for monitoring homologous recombination events.PloS one · 2021Article
- Effective identification of CRISPR/Cas9-induced and naturally occurred mutations in rice using a multiplex ligation-dependent probe amplification-based method.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Article
- Screening of the copy number increase ofInternational journal of clinical and experimental pathology · 2019Article
- qEva-CRISPR: a method for quantitative evaluation of CRISPR/Cas-mediated genome editing in target and off-target sites.Nucleic acids research · 2018Article
- The 30 kb deletion in theOncotarget · 2017Article
- MLPA-Based Analysis of Copy Number Variation in Plant Populations.Frontiers in plant science · 2017Article
- Article
- MTTE: an innovative strategy for the evaluation of targeted/exome enrichment efficiency.Oncotarget · 2016Article
- Copy number variation of genes involved in the hepatitis C virus-human interactome.Scientific reports · 2016Article
- Copy number variation in the region harboring SOX9 gene in dogs with testicular/ovotesticular disorder of sex development (78,XX; SRY-negative).Scientific reports · 2015Article
- High copy number variation of cancer-related microRNA genes and frequent amplification of DICER1 and DROSHA in lung cancer.Oncotarget · 2015Article
- Analysis of large mutations in BARD1 in patients with breast and/or ovarian cancer: the Polish population as an example.Scientific reports · 2015Article
- The use of a two-tiered testing strategy for the simultaneous detection of small EGFR mutations and EGFR amplification in lung cancer.PloS one · 2015Article
- Copy number variation of microRNA genes in the human genome.BMC genomics · 2011Article
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Authors and funding
4 authors.
Funding
Abstract
Multiplex ligation-dependent probe amplification (MLPA) is a multiplex copy number analysis method that is routinely used to identify large mutations in many clinical and research labs. One of the most important drawbacks of the standard MLPA setup is a complicated, and therefore expensive, procedure of generating long MLPA probes. This drawback substantially limits the applicability of MLPA to those genomic regions for which ready-to-use commercial kits are available. Here we present a simple protocol for designing MLPA probe sets that are composed entirely of short oligonucleotide half-probes generated through chemical synthesis. As an example, we present the design and generation of an MLPA assay for parallel copy number and small-mutation analysis of the EGFR gene.
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