ArticleFrontiers in neuroengineering2010
Human cell-based micro electrode array platform for studying neurotoxicity.
Article in Frontiers in neuroengineering, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- In vitro electrophysiological drug testing on neuronal networks derived from human induced pluripotent stem cells.Stem cell research & therapy · 2024Article
- Electrical and chemical modulation of homogeneous and heterogeneous human-iPSCs-derived neuronal networks on high density arrays.Frontiers in molecular neuroscience · 2024Article
- Review
- The Future of Neurotoxicology: A Neuroelectrophysiological Viewpoint.Frontiers in toxicology · 2021Article
- Toward Closed-Loop Electrical Stimulation of Neuronal Systems: A Review.Bioelectricity · 2020Review
- Innovations in 3-Dimensional Tissue Models of Human Brain Physiology and Diseases.Advanced functional materials · 2020Article
- Transparent Microelectrode Arrays Fabricated by Ion Beam Assisted Deposition for Neuronal Cell in Vitro Recordings.Micromachines · 2020Article
- Co-stimulation with IL-1β and TNF-α induces an inflammatory reactive astrocyte phenotype with neurosupportive characteristics in a human pluripotent stem cell model system.Scientific reports · 2019Article
- L-type voltage-gated calcium channel regulation of in vitro human cortical neuronal networks.Scientific reports · 2019Article
- Strategies to improve the regulatory assessment of developmental neurotoxicity (DNT) using in vitro methods.Toxicology and applied pharmacology · 2018Article
- Ion Beam Assisted E-Beam Deposited TiN Microelectrodes-Applied to Neuronal Cell Culture Medium Evaluation.Frontiers in neuroscience · 2018Article
- Optimised PDMS Tunnel Devices on MEAs Increase the Probability of Detecting Electrical Activity from Human Stem Cell-Derived Neuronal Networks.Frontiers in neuroscience · 2017Article
- Simulation of developing human neuronal cell networks.Biomedical engineering online · 2016Article
- Importance of being Nernst: Synaptic activity and functional relevance in stem cell-derived neurons.World journal of stem cells · 2015Review
- International STakeholder NETwork (ISTNET): creating a developmental neurotoxicity (DNT) testing road map for regulatory purposes.Archives of toxicology · 2015Article
- Establishment of a Long-Term Chick Forebrain Neuronal Culture on a Microelectrode Array Platform.RSC advances · 2015Article
- Biological and medical applications of a brain-on-a-chip.Experimental biology and medicine (Maywood, N.J.) · 2014Review
- Stem cells and stem cell-derived tissues and their use in safety assessment.The Journal of biological chemistry · 2014Review
- Healthy human CSF promotes glial differentiation of hESC-derived neural cells while retaining spontaneous activity in existing neuronal networks.Biology open · 2013Article
- Toward a 3D model of human brain development for studying gene/environment interactions.Stem cell research & therapy · 2013Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
At present, most of the neurotoxicological analyses are based on in vitro and in vivo models utilizing animal cells or animal models. In addition, the used in vitro models are mostly based on molecular biological end-point analyses. Thus, for neurotoxicological screening, human cell-based analysis platforms in which the functional neuronal networks responses for various neurotoxicants can be also detected real-time are highly needed. Microelectrode array (MEA) is a method which enables the measurement of functional activity of neuronal cell networks in vitro for long periods of time. Here, we utilize MEA to study the neurotoxicity of methyl mercury chloride (MeHgCl, concentrations 0.5-500 nM) to human embryonic stem cell (hESC)-derived neuronal cell networks exhibiting spontaneous electrical activity. The neuronal cell cultures were matured on MEAs into networks expressing spontaneous spike train-like activity before exposing the cells to MeHgCl for 72 h. MEA measurements were performed acutely and 24, 48, and 72 h after the onset of the exposure. Finally, exposed cells were analyzed with traditional molecular biological methods for cell proliferation, cell survival, and gene and protein expression. Our results show that 500 nM MeHgCl decreases the electrical signaling and alters the pharmacologic response of hESC-derived neuronal networks in delayed manner whereas effects can not be detected with qRT-PCR, immunostainings, or proliferation measurements. Thus, we conclude that human cell-based MEA platform is a sensitive online method for neurotoxicological screening.
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