ArticleNature methods2010
Dual RMCE for efficient re-engineering of mouse mutant alleles.
Article in Nature methods, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed.
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- TRACR: an anterograde transneuronal tracing system for genetic access across synapses and longitudinal circuit analysis.bioRxiv : the preprint server for biology · 2026Article
- A transposase-derived gene required for human brain development.Science advances · 2026Article
- Directed evolution on compact landing pads yields highly efficient recombinases for large DNA integration.Nucleic acids research · 2026Article
- PACAP38/mast-cell-specific receptor axis mediates repetitive stress-induced headache in mice.The journal of headache and pain · 2024Article
- Large T antigen mediated target gene replication improves site-specific recombination efficiency.Frontiers in bioengineering and biotechnology · 2024Article
- High-fidelity large-diversity monoclonal mammalian cell libraries by cell cycle arrested recombinase-mediated cassette exchange.Nucleic acids research · 2023Article
- Artificial chromosome technology and its potential application in plants.Frontiers in plant science · 2022Review
- Murine SEC24D can substitute functionally for SEC24C during embryonic development.Scientific reports · 2021Article
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- Genome targeting by hybrid Flp-TAL recombinases.Scientific reports · 2020Article
- Multidimensional imaging of liver injury repair in mice reveals fundamental role of the ductular reaction.Communications biology · 2020Article
- Renoprotective and Immunomodulatory Effects of GDF15 following AKI Invoked by Ischemia-Reperfusion Injury.Journal of the American Society of Nephrology : JASN · 2020Article
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- Smooth muscle cytochrome b5 reductase 3 deficiency accelerates pulmonary hypertension development in sickle cell mice.Blood advances · 2019Article
- Tau interactome analyses in CRISPR-Cas9 engineered neuronal cells reveal ATPase-dependent binding of wild-type but not P301L Tau to non-muscle myosins.Scientific reports · 2019Article
- Genes A27L and F13L as Genetic Markers for the Isolation of Recombinant Vaccinia Virus.Scientific reports · 2019Article
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6 authors.
Funding
Abstract
We have developed dual recombinase-mediated cassette exchange (dRMCE) to efficiently re-engineer the thousands of available conditional alleles in mouse embryonic stem cells. dRMCE takes advantage of the wild-type loxP and FRT sites present in these conditional alleles and in many gene-trap lines. dRMCE is a scalable, flexible tool to introduce tags, reporters and mutant coding regions into an endogenous locus of interest in an easy and highly efficient manner.
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