ArticleApplied and environmental microbiology2010
Characterization of the protocatechuate 4,5-cleavage pathway operon in Comamonas sp. strain E6 and discovery of a novel pathway gene.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 62 citations in OpenAlex.
- A 2-pyrone-4,6-dicarboxylic acid biosensor for lignin valorization based on a newly identified transcriptional regulatory system.Biotechnology for biofuels and bioproducts · 2026Article
- Genomic and functional insights into aromatic compound degradation byMicrobiology spectrum · 2026Article
- Microbial plastic degradation: enzymes, pathways, challenges, and perspectives.Microbiology and molecular biology reviews : MMBR · 2025Review
- Genetically encoded biosensor enabled mining, characterisation and engineering of aromatic acid MFS transporters.Journal of biological engineering · 2025Article
- Catabolic System of Syringic Acid, a Key Intermediate of Lignin-Derived Aromatic Compounds, via a Novel Linear Pathway inJournal of agricultural and food chemistry · 2025Article
- Environmental Impact of Xenobiotic Aromatic Compounds and Their Biodegradation Potential inInternational journal of molecular sciences · 2024Review
- Biodegradation of phthalic acid and terephthalic acid by Comamonas testosteroni strains.Folia microbiologica · 2024Article
- Engineering Comamonas testosteroni for the production of 2-pyrone-4,6-dicarboxylic acid as a promising building block.Microbial cell factories · 2023Article
- Deconstructed Plastic Substrate Preferences of Microbial Populations from the Natural Environment.Microbiology spectrum · 2023Article
- Characterization of Protocatechuate 4,5-Dioxygenase fromInternational journal of molecular sciences · 2021Article
- Distinct Changes Occur in the Human Breast Milk Microbiome Between Early and Established Lactation in Breastfeeding Guatemalan Mothers.Frontiers in microbiology · 2021Article
- Synergistic biodegradation of aromatic-aliphatic copolyester plastic by a marine microbial consortium.Nature communications · 2020Article
- Biotransformation of Monocyclic Phenolic Compounds byMicroorganisms · 2019Article
- Mapping the diversity of microbial lignin catabolism: experiences from the eLignin database.Applied microbiology and biotechnology · 2019Review
- Recent advances in lignin valorization with bacterial cultures: microorganisms, metabolic pathways, and bio-products.Biotechnology for biofuels · 2019Review
- The XylS/Pm regulator/promoter system and its use in fundamental studies of bacterial gene expression, recombinant protein production and metabolic engineering.Microbial biotechnology · 2017Review
- Influence of dairy by-product waste milk on the microbiomes of different gastrointestinal tract components in pre-weaned dairy calves.Scientific reports · 2017Article
- Structural and Kinetic Characterization of the 4-Carboxy-2-hydroxymuconate Hydratase from the Gallate and Protocatechuate 4,5-Cleavage Pathways of Pseudomonas putida KT2440.The Journal of biological chemistry · 2016Article
- Membrane-associated glucose-methanol-choline oxidoreductase family enzymes PhcC and PhcD are essential for enantioselective catabolism of dehydrodiconiferyl alcohol.Applied and environmental microbiology · 2015Article
- Methanogenic degradation of lignin-derived monoaromatic compounds by microbial enrichments from rice paddy field soil.Scientific reports · 2015Article
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The protocatechuate (PCA) 4,5-cleavage (PCA45) pathway is the essential catabolic route for the degradation of various aromatic acids in the genus Comamonas. All of the PCA45 pathway genes, orf1-pmdKEFDABC, as well as another PCA 4,5-dioxygenase gene, pmdA(II)B(II), were isolated from a phthalate-degrading bacterium, Comamonas sp. strain E6. Disruption of pmdB and pmdD in E6, which code for the β subunit of PCA 4,5-dioxygenase and 2-pyrone-4,6-dicarboxylate (PDC) hydrolase, respectively, resulted in a growth defect on PCA, indicating that these genes are essential for the growth of E6 on PCA. On the other hand, inactivation of pmdB(II) did not affect the growth of E6 on PCA. Disruption of pmdK, which is related to a 4-hydroxybenzoate/PCA transporter of Pseudomonas putida, resulted in growth retardation on PCA. The insertional inactivation of orf1 in E6, whose deduced amino acid sequence has no similarity with proteins of known function, led to the complete loss of growth on PCA and the accumulation of PDC and 4-oxalomesaconate (OMA) from PCA. These results indicated the involvement of orf1 in the PCA45 pathway, and this gene, designated pmdU, was suggested to code for OMA tautomerase. Reverse transcription-PCR analysis suggested that the pmdUKEFDABC genes constitute an operon. The transcription start site of the pmd operon was mapped at 167 nucleotides upstream of the initiation codon of pmdU. The pmd promoter activity was enhanced 20-fold when the cells were grown in the presence of PCA. Inducers of the pmd operon were found to be PCA and PDC, but PDC was the more effective inducer.
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