ArticleJournal of bacteriology2010
Biochemical characterization of individual components of the Allochromatium vinosum DsrMKJOP transmembrane complex aids understanding of complex function in vivo.
Article in Journal of bacteriology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Evidence for autotrophic growth of purple sulfur bacteria using pyrite as electron and sulfur source.Applied and environmental microbiology · 2024Article
- DsrMKJOP is the terminal reductase complex in anaerobic sulfate respiration.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Methylotrophic methanogenesis in the Archaeoglobi revealed by cultivation of Ca. Methanoglobus hypatiae from a Yellowstone hot spring.The ISME journal · 2024Article
- Stepwise pathway for early evolutionary assembly of dissimilatory sulfite and sulfate reduction.The ISME journal · 2023Article
- Integration of text mining and biological network analysis: Identification of essential genes in sulfate-reducing bacteria.Frontiers in microbiology · 2023Article
- Generation and Physiology of Hydrogen Sulfide and Reactive Sulfur Species in Bacteria.Antioxidants (Basel, Switzerland) · 2022Review
- Article
- The Ion-Translocating NrfD-Like Subunit of Energy-Transducing Membrane Complexes.Frontiers in chemistry · 2021Article
- Metabolic functions of the human gut microbiota: the role of metalloenzymes.Natural product reports · 2019Review
- Genomes of Neutrophilic Sulfur-Oxidizing Chemolithoautotrophs Representing 9 Proteobacterial Species From 8 Genera.Frontiers in microbiology · 2019Article
- Complete genome sequence of "Standards in genomic sciences · 2018Article
- The life sulfuric: microbial ecology of sulfur cycling in marine sediments.Environmental microbiology reports · 2017Review
- New proteins involved in sulfur trafficking in the cytoplasm of Allochromatium vinosum.The Journal of biological chemistry · 2014Article
- Metagenomic insights into S(0) precipitation in a terrestrial subsurface lithoautotrophic ecosystem.Frontiers in microbiology · 2014Article
- The genetic basis of energy conservation in the sulfate-reducing bacterium Desulfovibrio alaskensis G20.Frontiers in microbiology · 2014Article
- Identification of key components in the energy metabolism of the hyperthermophilic sulfate-reducing archaeon Archaeoglobus fulgidus by transcriptome analyses.Frontiers in microbiology · 2014Article
- Article
- Genome-wide transcriptional profiling of the purple sulfur bacterium Allochromatium vinosum DSM 180T during growth on different reduced sulfur compounds.Journal of bacteriology · 2013Article
- The bacterial SoxAX cytochromes.Cellular and molecular life sciences : CMLS · 2013Review
- Cytoplasmic sulfurtransferases in the purple sulfur bacterium Allochromatium vinosum: evidence for sulfur transfer from DsrEFH to DsrC.PloS one · 2012Article
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3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The DsrMKJOP transmembrane complex has a most important function in dissimilatory sulfur metabolism and consists of cytoplasmic, periplasmic, and membrane integral proteins carrying FeS centers and b- and c-type cytochromes as cofactors. In this study, the complex was isolated from the purple sulfur bacterium Allochromatium vinosum and individual components were characterized as recombinant proteins. The two integral membrane proteins DsrM and DsrP were successfully produced in Escherichia coli C43(DE3) and C41(DE3), respectively. DsrM was identified as a diheme cytochrome b, and the two hemes were found to be in low-spin state. Their midpoint redox potentials were determined to be +60 and +110 mV. Although no hemes were predicted for DsrP, it was also clearly identified as a b-type cytochrome. To the best of our knowledge, this is the first time that heme binding has been experimentally proven for a member of the NrfD protein family. Both cytochromes were partly reduced after addition of a menaquinol analogue, suggesting interaction with quinones in vivo. DsrO and DsrK were both experimentally proven to be FeS-containing proteins. In addition, DsrK was shown to be membrane associated, and we propose a monotopic membrane anchoring for this protein. Coelution assays provide support for the proposed interaction of DsrK with the soluble cytoplasmic protein DsrC, which might be its substrate. A model for the function of DsrMKJOP in the purple sulfur bacterium A. vinosum is presented.
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