ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2011
Multiple levels of gene regulation mediate differentiation of the intracellular pathogen Leishmania.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers.
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Who cites it
78 citing papers in PubMed, 143 citations in OpenAlex.
- Chromosome-level genome assembly of trypanosomatid parasite Lotmaria passim links chromosome duplication and divergence with infection of honey bees.BMC genomics · 2025Article
- A short ncRNA modulates gene expression and affects stress response and parasite differentiation inFrontiers in cellular and infection microbiology · 2025Article
- A divergent protein kinase A regulatory subunit essential for morphogenesis of the human pathogen Leishmania.PLoS pathogens · 2024Article
- LeishMANIAdb: a comparative resource for Leishmania proteins.Database : the journal of biological databases and curation · 2023Article
- Article
- The paradigm of intracellular parasite survival and drug resistance in leishmanial parasite through genome plasticity and epigenetics: Perception and future perspective.Frontiers in cellular and infection microbiology · 2023Review
- Identification of protein profile in metacyclic and amastigote-like stages of Leishmania tropica: a proteomic approach.AMB Express · 2022Article
- Stable Episomal TransfectantPathogens (Basel, Switzerland) · 2022Article
- Reporter gene systems: A powerful tool forCurrent research in microbial sciences · 2022Article
- Three types ofFrontiers in cellular and infection microbiology · 2022Article
- Finding Correlations Between mRNA and Protein Levels inFrontiers in cellular and infection microbiology · 2022Review
- Life Cycle Stage-Specific Accessibility of Leishmania donovani Chromatin at Transcription Start Regions.mSystems · 2021Article
- Quantitative Proteomics Reveals that Hsp90 Inhibition Dynamically Regulates Global Protein Synthesis in Leishmania mexicana.mSystems · 2021Article
- Heat Shock Proteins as the Druggable Targets in Leishmaniasis: Promises and Perils.Infection and immunity · 2021Review
- Lysosome Sensing Is a Key Mechanism inFrontiers in microbiology · 2021Review
- Advances in UnderstandingFrontiers in cell and developmental biology · 2021Review
- Transcriptome analysis of alternative splicing in the pathogen life cycle in human foreskin fibroblasts infected with Trypanosoma cruzi.Scientific reports · 2020Article
- TheGenes · 2020Article
- In-depth quantitative proteomics uncovers specie-specific metabolic programs in Leishmania (Viannia) species.PLoS neglected tropical diseases · 2020Article
- A discrete subtype of neural progenitor crucial for cortical folding in the gyrencephalic mammalian brain.eLife · 2020Article
18 more citing papers are in PubMed but not listed here.
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Authors and funding
11 authors at 5 institutions in 4 countries.
Funding
Abstract
For many years, mRNA abundance has been used as the surrogate measure of gene expression in biological systems. However, recent genome-scale analyses in both bacteria and eukaryotes have revealed that mRNA levels correlate with steady-state protein abundance for only 50-70% of genes, indicating that translation and post-translation processes also play important roles in determining gene expression. What is not yet clear is whether dynamic processes such as cell cycle progression, differentiation, or response to environmental changes change the relationship between mRNA and protein abundance. Here, we describe a systems approach to interrogate promastigote-to-amastigote differentiation in the obligatory intracellular parasitic protozoan Leishmania donovani. Our results indicate that regulation of mRNA levels plays a major role early in the differentiation process, while translation and post-translational regulation are more important in the latter part. In addition, it appears that the differentiation signal causes a transient global increase in the rate of protein synthesis, which is subsequently down-regulated by phosphorylation of α-subunit of translation initiation factor 2. Thus, Leishmania dynamically changes the relationship between mRNA and protein abundance as it adapts to new environmental circumstances. It is likely that similar mechanisms play a more important role than previously recognized in regulation of gene expression in other organisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.